Enhanced electrohydrodynamic collapse of DNA due to dilute polymers

Author(s)
Du, Ning; Doyle, Patrick S.; Renner, C. Benjamin
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Abstract
We experimentally demonstrate that addition of small, charge-neutral polymers to a buffer solution can promote compression of dilute solutions of single electrophoresing DNA. This phenomenon contrasts with the observed extension of DNA during capillary electrophoresis in dilute solutions of high molecular weight polymers. We propose these discrepancies in micron-scale DNA configurations arise from different nano-scale DNA-polymer collision events, controlled by solute polymer properties. We build upon theories previously proposed for intermolecular DNA aggregation in polymer-free solutions to develop scaling theories that describe trends seen in our data for intramolecular DNA compaction in dilute polymer solutions.
Date issued
2014-05
URI
http://hdl.handle.net/1721.1/101172
Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Journal
Biomicrofluidics
Publisher
American Institute of Physics (AIP)
Citation
Benjamin Renner, C., Ning Du, and Patrick S. Doyle. “Enhanced Electrohydrodynamic Collapse of DNA Due to Dilute Polymers.” Biomicrofluidics 8, no. 3 (May 2014): 034103. © 2014 AIP Publishing LLC
Version: Final published version
ISSN
1932-1058
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