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dc.contributor.authorHuang, Beijing K.
dc.contributor.authorSikes, Hadley
dc.date.accessioned2016-02-23T00:00:33Z
dc.date.available2016-02-23T00:00:33Z
dc.date.issued2014-08
dc.date.submitted2014-07
dc.identifier.issn22132317
dc.identifier.urihttp://hdl.handle.net/1721.1/101232
dc.description.abstractMolecular level, mechanistic understanding of the roles of reactive oxygen species (ROS) in a variety of pathological conditions is hindered by the difficulties associated with determining the concentration of various ROS species. Here, we present an approach that converts fold-change in the signal from an intracellular sensor of hydrogen peroxide into changes in absolute concentration. The method uses extracellular additions of peroxide and an improved biochemical measurement of the gradient between extracellular and intracellular peroxide concentrations to calibrate the intracellular sensor. By measuring peroxiredoxin activity, we found that this gradient is 650-fold rather than the 7–10-fold that is widely cited. The resulting calibration is important for understanding the mass-action kinetics of complex networks of redox reactions, and it enables meaningful characterization and comparison of outputs from endogenous peroxide generating tools and therapeutics across studies.en_US
dc.description.sponsorshipNational Science Foundation (U.S.). Graduate Research Fellowship (2009085738)en_US
dc.description.sponsorshipBurroughs Wellcome Fund (Career Award at the Scientific Interface 1005762)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.redox.2014.08.001en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en_US
dc.sourceElsevier Open Accessen_US
dc.titleQuantifying intracellular hydrogen peroxide perturbations in terms of concentrationen_US
dc.typeArticleen_US
dc.identifier.citationHuang, Beijing K., and Hadley D. Sikes. “Quantifying Intracellular Hydrogen Peroxide Perturbations in Terms of Concentration.” Redox Biology 2 (2014): 955–962.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorHuang, Beijing K.en_US
dc.contributor.mitauthorSikes, Hadleyen_US
dc.relation.journalRedox Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHuang, Beijing K.; Sikes, Hadley D.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-9238-8932
dc.identifier.orcidhttps://orcid.org/0000-0002-7096-138X
mit.licensePUBLISHER_CCen_US


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