DNA Assembly in 3D Printed Fluidics
Author(s)Patrick, William G.; Levy, Taylor J.; Wang, Che-Wei; Rivera, Jaime J.; Voigt, Christopher A.; Oxman, Neri; Kong, David S.; Nielsen, Alec Andrew; Keating, Steven John; Mondragon-Palomino, Octavio; Carr, Peter A., Sr.; ... Show more Show less
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The process of connecting genetic parts—DNA assembly—is a foundational technology for synthetic biology. Microfluidics present an attractive solution for minimizing use of costly reagents, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication and operation can be expensive and requires expertise, limiting access to the technology. With advances in commodity digital fabrication tools, it is now possible to directly print fluidic devices and supporting hardware. 3D printed micro- and millifluidic devices are inexpensive, easy to make and quick to produce. We demonstrate Golden Gate DNA assembly in 3D-printed fluidics with reaction volumes as small as 490 nL, channel widths as fine as 220 microns, and per unit part costs ranging from $0.61 to $5.71. A 3D-printed syringe pump with an accompanying programmable software interface was designed and fabricated to operate the devices. Quick turnaround and inexpensive materials allowed for rapid exploration of device parameters, demonstrating a manufacturing paradigm for designing and fabricating hardware for synthetic biology.
DepartmentLincoln Laboratory; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Media Laboratory
Public Library of Science
Patrick, William G., Alec A. K. Nielsen, Steven J. Keating, Taylor J. Levy, Che-Wei Wang, Jaime J. Rivera, Octavio Mondragon-Palomino, et al. “DNA Assembly in 3D Printed Fluidics.” Edited by Meni Wanunu. PLoS ONE 10, no. 12 (December 30, 2015): e0143636.
Final published version