Single-cell and metagenomic analyses indicate a fermentative and saccharolytic lifestyle for members of the OP9 lineage
Author(s)
Dodsworth, Jeremy A.; Blainey, Paul C.; Murugapiran, Senthil K.; Swingley, Wesley D.; Ross, Christian A.; Tringe, Susannah G.; Chain, Patrick S. G.; Scholz, Matthew B.; Lo, Chien-Chi; Raymond, Jason; Quake, Stephen R.; Hedlund, Brian P.; ... Show more Show less
DownloadRoss_Single-cell.pdf (1.934Mb)
OPEN_ACCESS_POLICY
Open Access Policy
Creative Commons Attribution-Noncommercial-Share Alike
Terms of use
Metadata
Show full item recordAbstract
OP9 is a yet-uncultivated bacterial lineage found in geothermal systems, petroleum reservoirs, anaerobic digesters and wastewater treatment facilities. Here we use single-cell and metagenome sequencing to obtain two distinct, nearly complete OP9 genomes, one constructed from single cells sorted from hot spring sediments and the other derived from binned metagenomic contigs from an in situ-enriched cellulolytic, thermophilic community. Phylogenomic analyses support the designation of OP9 as a candidate phylum for which we propose the name ‘Atribacteria’. Although a plurality of predicted proteins is most similar to those from Firmicutes, the presence of key genes suggests a diderm cell envelope. Metabolic reconstruction from the core genome suggests an anaerobic lifestyle based on sugar fermentation by Embden–Meyerhof glycolysis with production of hydrogen, acetate and ethanol. Putative glycohydrolases and an endoglucanase may enable catabolism of (hemi)cellulose in thermal environments. This study lays a foundation for understanding the physiology and ecological role of the ‘Atribacteria’.
Date issued
2013-05Department
Massachusetts Institute of Technology. Department of Biological EngineeringJournal
Nature Communications
Publisher
Nature Publishing Group
Citation
Dodsworth, Jeremy A., Paul C. Blainey, Senthil K. Murugapiran, Wesley D. Swingley, Christian A. Ross, Susannah G. Tringe, Patrick S. G. Chain, et al. “Single-Cell and Metagenomic Analyses Indicate a Fermentative and Saccharolytic Lifestyle for Members of the OP9 Lineage.” Nat Comms 4 (May 14, 2013): 1854.
Version: Author's final manuscript
ISSN
2041-1723