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Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease

dc.contributor.authorJoung, Julia
dc.contributor.authorZhang, Feng
dc.contributor.authorKonermann, Silvana
dc.contributor.authorDahlman, James E.
dc.contributor.authorAbudayyeh, Omar Osama
dc.contributor.authorGootenberg, Jonathan S.
dc.date.accessioned2016-05-22T23:41:33Z
dc.date.available2016-05-22T23:41:33Z
dc.date.issued2015-10
dc.date.submitted2015-08
dc.identifier.issn1087-0156
dc.identifier.issn1546-1696
dc.identifier.urihttp://hdl.handle.net/1721.1/102583
dc.description.abstractWe have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.en_US
dc.description.sponsorshipMcGovern Institute for Brain Research at MIT (Friends of McGovern Institute Fellowship)en_US
dc.description.sponsorshipUnited States. Dept. of Energy (Computational Science Graduate Fellowship)en_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (1DP1-MH100706)en_US
dc.description.sponsorshipPoitras Foundationen_US
dc.description.sponsorshipVallee Foundationen_US
dc.description.sponsorshipSimons Foundationen_US
dc.description.sponsorshipPaul G. Allen Foundationen_US
dc.description.sponsorshipNew York Stem Cell Foundationen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nbt.3390en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleOrthogonal gene knockout and activation with a catalytically active Cas9 nucleaseen_US
dc.typeArticleen_US
dc.identifier.citationDahlman, James E, Omar O Abudayyeh, Julia Joung, Jonathan S Gootenberg, Feng Zhang, and Silvana Konermann. “Orthogonal Gene Knockout and Activation with a Catalytically Active Cas9 Nuclease.” Nature Biotechnology 33, no. 11 (October 5, 2015): 1159–61.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.mitauthorAbudayyeh, Omar Osamaen_US
dc.contributor.mitauthorJoung, Juliaen_US
dc.contributor.mitauthorGootenberg, Jonathan S.en_US
dc.contributor.mitauthorZhang, Fengen_US
dc.contributor.mitauthorKonermann, Silvanaen_US
dc.relation.journalNature Biotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsDahlman, James E; Abudayyeh, Omar O; Joung, Julia; Gootenberg, Jonathan S; Zhang, Feng; Konermann, Silvanaen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2782-2509
dc.identifier.orcidhttps://orcid.org/0000-0001-7915-1685
dc.identifier.orcidhttps://orcid.org/0000-0001-6656-5002
dc.identifier.orcidhttps://orcid.org/0000-0002-7979-3220
mit.licenseOPEN_ACCESS_POLICYen_US


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