| dc.contributor.author | Barkal, Amira A. | |
| dc.contributor.author | Srinivasan, Sharanya | |
| dc.contributor.author | Gifford, David K. | |
| dc.contributor.author | Sherwood, Richard I. | |
| dc.contributor.author | Hashimoto, Tatsunori Benjamin | |
| dc.date.accessioned | 2016-05-23T14:44:15Z | |
| dc.date.available | 2016-05-23T14:44:15Z | |
| dc.date.issued | 2016-03 | |
| dc.date.submitted | 2015-10 | |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/102604 | |
| dc.description.abstract | Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromatin accessibility to alter local transcription factor binding. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (5UL1DE019581) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (RL1DE019021) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (1K01DK101684-01) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (1U01HG007037) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (5P01NS055923) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Public Library of Science | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1371/journal.pone.0152683 | en_US |
| dc.rights | Creative Commons Attribution | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
| dc.source | Public Library of Science | en_US |
| dc.title | Cas9 Functionally Opens Chromatin | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Barkal, Amira A., Sharanya Srinivasan, Tatsunori Hashimoto, David K. Gifford, and Richard I. Sherwood. “Cas9 Functionally Opens Chromatin.” Edited by Hodaka Fujii. PLOS ONE 11, no. 3 (March 31, 2016): e0152683. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
| dc.contributor.mitauthor | Barkal, Amira A. | en_US |
| dc.contributor.mitauthor | Hashimoto, Tatsunori Benjamin | en_US |
| dc.contributor.mitauthor | Gifford, David K. | en_US |
| dc.relation.journal | PLOS ONE | en_US |
| dc.eprint.version | Final published version | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Barkal, Amira A.; Srinivasan, Sharanya; Hashimoto, Tatsunori; Gifford, David K.; Sherwood, Richard I. | en_US |
| dspace.embargo.terms | N | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0003-0521-5855 | |
| dc.identifier.orcid | https://orcid.org/0000-0003-1709-4034 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
