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dc.contributor.authorHaws, Michael E.
dc.contributor.authorJaramillo, Thomas C.
dc.contributor.authorEspinosa, Felipe
dc.contributor.authorJ. Widman, Allie
dc.contributor.authorStuber, Garret D.
dc.contributor.authorSparta, Dennis R.
dc.contributor.authorRusso, Scott J.
dc.contributor.authorParada, Luis F.
dc.contributor.authorStavarache, Mihaela
dc.contributor.authorKaplitt, Michael
dc.contributor.authorBonci, Antonello
dc.contributor.authorPowell, Craig M.
dc.contributor.authorTye, Kay
dc.date.accessioned2016-05-24T23:54:56Z
dc.date.available2016-05-24T23:54:56Z
dc.date.issued2013-11
dc.date.submitted2013-10
dc.identifier.issn00219967
dc.identifier.issn1096-9861
dc.identifier.urihttp://hdl.handle.net/1721.1/102670
dc.description.abstractMutations in phosphatase and tensin homolog deleted on chromosome 10 (PTEN) are implicated in neuropsychiatric disorders including autism. Previous studies report that PTEN knockdown in neurons in vivo leads to increased spine density and synaptic activity. To better characterize synaptic changes in neurons lacking PTEN, we examined the effects of shRNA knockdown of PTEN in basolateral amygdala neurons on synaptic spine density and morphology by using fluorescent dye confocal imaging. Contrary to previous studies in the dentate gyrus, we find that knockdown of PTEN in basolateral amygdala leads to a significant decrease in total spine density in distal dendrites. Curiously, this decreased spine density is associated with increased miniature excitatory postsynaptic current frequency and amplitude, suggesting an increase in number and function of mature spines. These seemingly contradictory findings were reconciled by spine morphology analysis demonstrating increased mushroom spine density and size with correspondingly decreased thin protrusion density at more distal segments. The same analysis of PTEN conditional deletion in the dentate gyrus demonstrated that loss of PTEN does not significantly alter total density of dendritic protrusions in the dentate gyrus, but does decrease thin protrusion density and increases density of more mature mushroom spines. These findings suggest that, contrary to previous reports, PTEN knockdown may not induce de novo spinogenesis, but instead may increase synaptic activity by inducing morphological and functional maturation of spines. Furthermore, behavioral analysis of basolateral amygdala PTEN knockdown suggests that these changes limited only to the basolateral amygdala complex may not be sufficient to induce increased anxiety-related behaviors.en_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/cne.23488en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titlePTEN knockdown alters dendritic spine/protrusion morphology, not densityen_US
dc.typeArticleen_US
dc.identifier.citationHaws, Michael E., Thomas C. Jaramillo, Felipe Espinosa, Allie J. Widman, Garret D. Stuber, Dennis R. Sparta, Kay M. Tye, et al. “PTEN Knockdown Alters Dendritic Spine/protrusion Morphology, Not Density.” J. Comp. Neurol. 522, no. 5 (February 12, 2014): 1171–1190.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.mitauthorTye, Kayen_US
dc.relation.journalJournal of Comparative Neurologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHaws, Michael E.; Jaramillo, Thomas C.; Espinosa, Felipe; J. Widman, Allie; Stuber, Garret D.; Sparta, Dennis R.; Tye, Kay M.; Russo, Scott J.; Parada, Luis F.; Stavarache, Mihaela; Kaplitt, Michael; Bonci, Antonello; Powell, Craig M.en_US
dspace.embargo.termsNen_US
mit.licenseOPEN_ACCESS_POLICYen_US


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