Iso-acoustic focusing of cells for size-insensitive acousto-mechanical phenotyping
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ncomms11556.pdf
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Author(s)
Augustsson, Per
Karlsen, Jonas T.
Su, Hao-Wei
Bruus, Henrik
Voldman, Joel
Date Issued
May 2016
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Augustsson, Per, Jonas T. Karlsen, Hao-Wei Su, Henrik Bruus, and Joel Voldman. “Iso-Acoustic Focusing of Cells for Size-Insensitive Acousto-Mechanical Phenotyping.” Nat Comms 7 (May 16, 2016): 11556.
Version
Final published version
Abstract
Mechanical phenotyping of single cells is an emerging tool for cell classification, enabling assessment of effective parameters relating to cells’ interior molecular content and structure. Here, we present iso-acoustic focusing, an equilibrium method to analyze the effective acoustic impedance of single cells in continuous flow. While flowing through a microchannel, cells migrate sideways, influenced by an acoustic field, into streams of increasing acoustic impedance, until reaching their cell-type specific point of zero acoustic contrast. We establish an experimental procedure and provide theoretical justifications and models for iso-acoustic focusing. We describe a method for providing a suitable acoustic contrast gradient in a cell-friendly medium, and use acoustic forces to maintain that gradient in the presence of destabilizing forces. Applying this method we demonstrate iso-acoustic focusing of cell lines and leukocytes, showing that acoustic properties provide phenotypic information independent of size.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1038/ncomms11556
