Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator
Author(s)Wong, Yoke-Ming; Brigham, Christopher J.; Rha, ChoKyun; Sinskey, Anthony J.; Sudesh, Kumar
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The potential of plant oils as sole carbon sources for production of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction using the recombinant Cupriavidus necator strain Re2160/pCB113 has been investigated. Various types and concentrations of plant oils were evaluated for efficient conversion of P(3HB-co-3HHx) copolymer. Crude palm kernel oil (CPKO) at a concentration of 2.5 g/L was found to be most suitable for production of copolymer with a 3HHx content of approximately 70 mol%. The time profile of these cells was also examined in order to study the trend of 3HHx monomer incorporation, PHA production and PHA synthase activity. [superscript 1]H NMR and [superscript 13]C NMR analyses confirmed the presence of P(3HB-co-3HHx) copolymer containing a high 3HHx monomer fraction, in which monomers were not randomly distributed. The results of various characterization analyses revealed that the copolymers containing a high 3HHx monomer fraction demonstrated soft and flexible mechanical properties.
DepartmentHarvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Biomaterials Science and Engineering Laboratory; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Engineering Systems Division
Wong, Yoke-Ming, Christopher J. Brigham, ChoKyun Rha, Anthony J. Sinskey, and Kumar Sudesh. “Biosynthesis and Characterization of Polyhydroxyalkanoate Containing High 3-Hydroxyhexanoate Monomer Fraction from Crude Palm Kernel Oil by Recombinant Cupriavidus Necator.” Bioresource Technology 121 (October 2012): 320–327.
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