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dc.contributor.authorSabino, C. P.
dc.contributor.authorGarcez, A. S.
dc.contributor.authorNúñez, S. C.
dc.contributor.authorRibeiro, M. S.
dc.contributor.authorHamblin, Michael R
dc.date.accessioned2016-09-15T22:16:08Z
dc.date.available2016-09-15T22:16:08Z
dc.date.issued2014-07
dc.date.submitted2013-10
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.urihttp://hdl.handle.net/1721.1/104339
dc.description.abstractAntimicrobial photodynamic therapy (APDT) combined with endodontic treatment has been recognized as an alternative approach to complement conventional root canal disinfection methods on bacterial biofilms. We developed an in vitro model of bioluminescent Candida albicans biofilm inside curved dental root canals and investigated the microbial reduction produced when different light delivery methods are employed. Each light delivery method was evaluated in respect to the light distribution provided inside curved root canals. After conventional endodontic preparation, teeth were sterilized before canals were contaminated by a bioluminescent strain of C. albicans (CEC789). Methylene blue (90 μM) was introduced into the canals and then irradiated (λ = 660 nm, P = 100 mW, beam diameter = 2 mm) with laser tip either in contact with pulp chamber or within the canal using an optical diffuser fiber. Light distribution was evaluated by CCD camera, and microbial reduction was monitored through bioluminescence imaging. Our findings demonstrated that the bioluminescent C. albicans biofilm model had good reproducibility and uniformity. Light distribution in dental tissue was markedly dependent on the light delivery system, and this strategy was directly related to microbial destruction. Both light delivery systems performed significant fungal inactivation. However, when irradiation was performed with optical diffuser fiber, microbial burden reduction was nearly 100 times more effective. Bioluminescence is an interesting real-time analysis to endodontic C. albicans biofilm inactivation. APDT showed to be an effective way to inactivate C. albicans biofilms. Diffuser fibers provided optimized light distribution inside curved root canals and significantly increased APDT efficiency.en_US
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grant 2010/13313-9)en_US
dc.description.sponsorshipConselho Nacional de Pesquisas (Brazil) Conselho Nacional de Desenvolvimento Cientifico e Tecnologicoen_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (NIH R01AI050875)en_US
dc.publisherSpringer Londonen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s10103-014-1629-xen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSpringer Londonen_US
dc.titleReal-time evaluation of two light delivery systems for photodynamic disinfection of Candida albicans biofilm in curved root canalsen_US
dc.typeArticleen_US
dc.identifier.citationSabino, C. P. et al. “Real-Time Evaluation of Two Light Delivery Systems for Photodynamic Disinfection of Candida Albicans Biofilm in Curved Root Canals.” Lasers in Medical Science 30.6 (2015): 1657–1665.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.mitauthorHamblin, Michael R
dc.relation.journalLasers in Medical Scienceen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2016-08-18T15:21:13Z
dc.language.rfc3066en
dc.rights.holderSpringer-Verlag London
dspace.orderedauthorsSabino, C. P.; Garcez, A. S.; Núñez, S. C.; Ribeiro, M. S.; Hamblin, M. R.en_US
dspace.embargo.termsNen
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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