Show simple item record

dc.contributor.authorD’Angelo, Paola A.
dc.contributor.authorBromberg, Lev E
dc.contributor.authorHatton, Trevor Alan
dc.contributor.authorWilusz, Eugene
dc.date.accessioned2016-09-21T19:34:17Z
dc.date.available2017-03-01T16:14:47Z
dc.date.issued2016-04
dc.date.submitted2016-03
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.urihttp://hdl.handle.net/1721.1/104362
dc.description.abstractWe report on the performance of brominated poly(N-vinylpyrrolidone) (PVP-Br), brominated poly(ethylene glycol) (PEG-Br), and brominated poly(allylamine-co-4-aminopyridine) (PAAm-APy-Br) for their ability to decontaminate Bacillus anthracis Sterne spores in solution while also allowing for the sensing of the spores. The polymers were brominated by bromine using carbon tetrachloride or potassium tribromide as solvents, with bromine loadings ranging from 1.6 to 4.2 mEq/g of polymer. B. anthracis Sterne spores were exposed to increasing concentrations of brominated polymers for 5 min, while the kinetics of the sporicidal activity was assessed. All brominated polymers demonstrated spore log-kills of 8 within 5 min of exposure at 12 mg/mL aqueous polymer concentration. Sensing of spores was accomplished by measuring the release of dipicolinic acid (DPA) from the spore using time-resolved fluorescence. Parent, non-brominated polymers did not cause any release of DPA and the spores remained viable. In contrast, spores exposed to the brominated polymers were inactivated and the release of DPA was observed within minutes of exposure. Also, this release of DPA continued for a long time after spore inactivation as in a controlled release process. The DPA release was more pronounced for spores exposed to brominated PVP and brominated PEG-8000 compared to brominated PAAm-APy and brominated PEG-400. Using time-resolved fluorescence, we detected as low as 2500 B. anthracis spores, with PEG-8000 being more sensitive to low spore numbers. Our results suggest that the brominated polymers may be used effectively as decontamination agents against bacterial spores while also providing the sensing capability.en_US
dc.description.sponsorshipDefense Threat Reduction Agency (DTRA)en_US
dc.publisherSpringer Berlin Heidelbergen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s00253-016-7507-7en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceSpringer Berlin Heidelbergen_US
dc.titleSensing and inactivation of Bacillus anthracis Sterne by polymer–bromine complexesen_US
dc.typeArticleen_US
dc.identifier.citationD’Angelo, Paola A., Lev Bromberg, T. Alan Hatton, and Eugene Wilusz. “Sensing and Inactivation of Bacillus Anthracis Sterne by Polymer–bromine Complexes.” Applied Microbiology and Biotechnology 100, no. 15 (April 18, 2016): 6847-6857.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorBromberg, Lev E
dc.contributor.mitauthorHatton, Trevor Alan
dc.relation.journalApplied Microbiology and Biotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2016-08-18T15:24:35Z
dc.language.rfc3066en
dc.rights.holderSpringer-Verlag Berlin Heidelberg (outside the USA)
dspace.embargo.termsNen
dc.identifier.orcidhttps://orcid.org/0000-0002-4558-245X
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record