Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha
Author(s)Jeon, Jong-Min; Kim, Yong-Hyun; Kim, Hyun-Joong; Yi, Da-Hye; Kim, Hyungsup; Rha, ChoKyun; Yang, Yung-Hun; Brigham, Christopher J.; Sinskey, Anthony J; ... Show more Show less
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Polyhydroxyalkanoates (PHAs), a promising family of bio-based polymers, are considered to be alternatives to traditional petroleum-based plastics. Copolymers like poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) have been shown to exhibit favorable physical and mechanical properties, due to decreased crystallinity resulting from the presence of medium-chain-length 3-hydroxyhexanoate (3HHx) monomers. In this study, we produced P(HB-co-HHx) using engineered Ralstonia eutropha strains containing deletions of the acetoacetyl-CoA reductase (phaB) genes and replacing the native PHA synthase with phaC2 from Rhodococcus aetherivorans I24 and by using butyrate, a short-chain organic acid, as the carbon source. Although the wild-type R. eutropha did not produce P(HB-co-HHx) when grown on mixed acids or on butyrate as the sole carbon source, we are able to produce polymer containing up to 40 wt% 3HHx monomer with the aforementioned engineered R. eutropha strains using various concentrations of just butyrate as the sole carbon source. This is the first report for the production of P(HB-co-HHx) copolymer in R. eutropha using butyrate.
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Engineering Systems Division
Applied Microbiology and Biotechnology
Springer Berlin Heidelberg
Jeon, Jong-Min et al. “Biosynthesis of poly(3-Hydroxybutyrate-Co-3-Hydroxyhexanoate) (P(HB-Co-HHx)) from Butyrate Using Engineered Ralstonia Eutropha.” Applied Microbiology and Biotechnology 98.12 (2014): 5461–5469.
Author's final manuscript