Show simple item record

dc.contributor.authorvan Vliet, Miranda
dc.contributor.authorConlon, Christine
dc.contributor.authorGrasso, Daniel J.
dc.contributor.authorBrooks, Daniel J.
dc.contributor.authorBouxsein, Mary L.
dc.contributor.authorEllman, Rachel
dc.contributor.authorSpatz, Jordan Matthew
dc.date.accessioned2016-10-28T16:19:26Z
dc.date.available2016-10-28T16:19:26Z
dc.date.issued2013-11
dc.date.submitted2013-07
dc.identifier.issn0171-967X
dc.identifier.issn1432-0827
dc.identifier.urihttp://hdl.handle.net/1721.1/105131
dc.description.abstractBone receives mechanical stimulation from two primary sources, muscle contractions and external gravitational loading; but the relative contribution of each source to skeletal health is not fully understood. Understanding the most effective loading for maintaining bone health has important clinical implications for prescribing physical activity for the treatment or prevention of osteoporosis. Therefore, we investigated the relative effects of muscle paralysis and reduced gravitational loading on changes in muscle mass, bone mineral density, and microarchitecture. Adult female C57Bl/6J mice (n = 10/group) underwent one of the following: unilateral botulinum toxin (BTX) injection of the hind limb, hind limb unloading (HLU), both unilateral BTX injection and HLU, or no intervention. BTX and HLU each led to significant muscle and bone loss. The effect of BTX was diminished when combined with HLU, though generally the leg that received the combined intervention (HLU+BTX) had the most detrimental changes in bone and muscle. We found an indirect effect of BTX affecting the uninjected (contralateral) leg that led to significant decreases in bone mineral density and deficits in muscle mass and bone architecture relative to the untreated controls; the magnitude of this indirect BTX effect was comparable to the direct effect of BTX treatment and HLU. Thus, while it was difficult to definitively conclude whether muscle force or external gravitational loading contributes more to bone maintenance, it appears that BTX-induced muscle paralysis is more detrimental to muscle and bone than HLU.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (H R21 AR057522)en_US
dc.description.sponsorshipUnited States. National Aeronautics and Space Administration (NNX10AE39G)en_US
dc.description.sponsorshipNational Space Biomedical Research Institute (NASA NCC 9-58)en_US
dc.description.sponsorshipUnited States. National Aeronautics and Space Administration (NASA-Jenkins predoctoral fellowship)en_US
dc.description.sponsorshipNorthrop Grumman Corporation (Aerospace Systems PhD Training Fellowship)en_US
dc.publisherSpringer USen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s00223-013-9814-7en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSpringer USen_US
dc.titleCombined Effects of Botulinum Toxin Injection and Hind Limb Unloading on Bone and Muscleen_US
dc.typeArticleen_US
dc.identifier.citationEllman, Rachel et al. “Combined Effects of Botulinum Toxin Injection and Hind Limb Unloading on Bone and Muscle.” Calcified Tissue International 94.3 (2014): 327–337.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.mitauthorEllman, Rachel
dc.contributor.mitauthorSpatz, Jordan Matthew
dc.relation.journalCalcified Tissue Internationalen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2016-08-18T15:41:02Z
dc.language.rfc3066en
dc.rights.holderSpringer Science+Business Media New York
dspace.orderedauthorsEllman, Rachel; Grasso, Daniel J.; van Vliet, Miranda; Brooks, Daniel J.; Spatz, Jordan M.; Conlon, Christine; Bouxsein, Mary L.en_US
dspace.embargo.termsNen
mit.licensePUBLISHER_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record