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dc.contributor.authorYoo, Byunghee
dc.contributor.authorKavishwar, Amol
dc.contributor.authorRoss, Alana
dc.contributor.authorPantazopoulos, Pamela
dc.contributor.authorMoore, Anna
dc.contributor.authorMedarova, Zdravka
dc.date.accessioned2016-11-07T18:18:45Z
dc.date.available2016-11-07T18:18:45Z
dc.date.issued2015-05
dc.identifier.issn1536-1632
dc.identifier.issn1860-2002
dc.identifier.urihttp://hdl.handle.net/1721.1/105229
dc.description.abstractPurpose The development of tools for the analysis of microRNA (miRNA) function in tumors can advance our diagnostic and prognostic capabilities. Here, we describe the development of technology for the profiling of miRNA expression in the tumors of live animals. Procedures The approach is based on miRNA nanosensors consisting of sensor oligonucleotides conjugated to magnetic nanoparticles for systemic delivery. Feasibility was demonstrated for the detection of miR-10b, implicated in epithelial to mesenchymal transition and the development of metastasis. The miR-10b nanosensor was tested in vivo in two mouse models of cancer. In the first model, mice were implanted subcutaneously with MDA-MB-231-luc-D3H2LN tumors, in which miR-10b was inhibited. In the second model, mice were implanted bilaterally with metastatic MDA-MB-231 and nonmetastatic MCF-7 cells. The nanosensors were injected intravenously, and fluorescence intensity in the tumors was monitored over time. Results We showed that the described nanosensors are capable of discriminating between tumors based on their expression of miR-10b. Radiant efficiency was higher in the miR-10b-active tumors than in the miR-10b-inhibited tumors and in the MDA-MB-231 tumors relative to the MCF-7 tumors. Conclusions The described technology provides an important tool that could be used to answer questions about microRNA function in cancer.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (National Cancer Institute R01CA16346101A1)en_US
dc.description.sponsorshipBreast Cancer Alliance (Young Investigator Award)en_US
dc.publisherSpringer USen_US
dc.relation.isversionofhttp://dx.doi.org/10.1007/s11307-015-0863-3en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSpringer USen_US
dc.titleIn Vivo Detection of miRNA Expression in Tumors Using an Activatable Nanosensoren_US
dc.typeArticleen_US
dc.identifier.citationYoo, Byunghee et al. “In Vivo Detection of miRNA Expression in Tumors Using an Activatable Nanosensor.” Molecular Imaging and Biology 18.1 (2016): 70–78.en_US
dc.contributor.departmentMartinos Imaging Center (McGovern Institute for Brain Research at MIT)en_US
dc.relation.journalMolecular Imaging and Biologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2016-08-18T15:45:08Z
dc.language.rfc3066en
dc.rights.holderWorld Molecular Imaging Society
dspace.orderedauthorsYoo, Byunghee; Kavishwar, Amol; Ross, Alana; Pantazopoulos, Pamela; Moore, Anna; Medarova, Zdravkaen_US
dspace.embargo.termsNen
mit.licensePUBLISHER_POLICYen_US


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