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dc.contributor.authorJohnson, Christopher M
dc.contributor.authorGrossman, Alan Davis
dc.date.accessioned2016-11-08T19:49:25Z
dc.date.available2016-11-08T19:49:25Z
dc.date.issued2014-08
dc.date.submitted2014-07
dc.identifier.issn1365-2958
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/1721.1/105268
dc.description.abstractConjugation, a major type of horizontal gene transfer in bacteria, involves transfer of DNA from a donor to a recipient using donor-encoded conjugation machinery. Using a high throughput screen (Tn-seq), we identified genes in recipients that contribute to acquisition of the integrative and conjugative element ICEBs1 by Bacillus subtilis. We found that null mutations in some genes caused an increase, and others a decrease in conjugation efficiency. Some mutations affected conjugation only when present in recipients. Other mutations affected conjugation when present in donors or recipients. Most of the genes identified are known or predicted to affect the cell envelope. Several encode enzymes involved in phospholipid biosynthesis and one encodes a homolog of penicillin binding proteins. Two of the genes identified also affected conjugation of Tn916, indicating that their roles in conjugation may be general. We did not identify any genes in recipients that were essential for ICEBs1 conjugation, indicating that if there are such genes, then these are either essential for cell growth or redundant. Our results indicate that acquisition of ICEBs1, and perhaps other conjugative elements, is robust and not easily avoided by mutation and that several membrane-related functions affect the efficiency of conjugationen_US
dc.description.sponsorshipNational Institute of General Medical Sciences (U.S.) (Award R01GM050895)en_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/mmi.12736en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleIdentification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilisen_US
dc.typeArticleen_US
dc.identifier.citationJohnson, Christopher M., and Alan D. Grossman. “Identification of Host Genes That Affect Acquisition of an Integrative and Conjugative Element in B Acillus Subtilis: Genes in Recipients That Affect Conjugation.” Molecular Microbiology 2014 September ; 93(6): 1284–1301.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Center for Environmental Health Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorJohnson, Christopher M
dc.contributor.mitauthorGrossman, Alan Davis
dc.relation.journalMolecular Microbiologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsJohnson, Christopher M.; Grossman, Alan D.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-2298-7462
dc.identifier.orcidhttps://orcid.org/0000-0002-8235-7227
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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