dc.contributor.author | Makarova, Kira S. | |
dc.contributor.author | van der Oost, John | |
dc.contributor.author | Koonin, Eugene V. | |
dc.contributor.author | Zetsche, Bernd | |
dc.contributor.author | Gootenberg, Jonathan S | |
dc.contributor.author | Abudayyeh, Omar Osama | |
dc.contributor.author | Slaymaker, Ian | |
dc.contributor.author | Essletzbichler, Patrick | |
dc.contributor.author | Volz, Sara E. | |
dc.contributor.author | Joung, Julia | |
dc.contributor.author | Regev, Aviv | |
dc.contributor.author | Zhang, Feng | |
dc.date.accessioned | 2016-12-07T21:38:01Z | |
dc.date.available | 2016-12-07T21:38:01Z | |
dc.date.issued | 2015-09 | |
dc.date.submitted | 2015-09 | |
dc.identifier.issn | 00928674 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/105747 | |
dc.description.abstract | The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multiprotein complexes, whereas Class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here we report characterization of Cpf1, a putative Class 2 CRISPR effector. We demonstrate that Cpf1 mediates robust DNA interference with features distinct from Cas9. Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer adjacent motif. Moreover, Cpf1 cleaves DNA via a staggered DNA double stranded break. Out of 16 Cpf1-family proteins, we identified two candidate enzymes, from Acidominococcus and Lachnospiraceae, with efficient genome editing activity in human cells. Identifying this mechanism of interference broadens our understanding of CRISPR-Cas systems
and advances their genome editing applications. | en_US |
dc.description.sponsorship | United States. Dept. of Energy (Computational Science Graduate Fellowship) | en_US |
dc.description.sponsorship | National Institute of Mental Health (U.S.) (Grant 1DP1-MH100706) | en_US |
dc.description.sponsorship | Poitras Foundation | en_US |
dc.description.sponsorship | Vallee Foundation | en_US |
dc.description.sponsorship | Simons Foundation | en_US |
dc.description.sponsorship | Paul G. Allen Family Foundation | en_US |
dc.description.sponsorship | New York Stem Cell Foundation | en_US |
dc.description.sponsorship | Robert Metcalfe | en_US |
dc.description.sponsorship | David R. Cheng | en_US |
dc.language.iso | en_US | |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1016/j.cell.2015.09.038 | en_US |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Zetsche, Bernd et al. “Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.” Cell 163.3 (2015): 759–771. | en_US |
dc.contributor.department | Harvard University--MIT Division of Health Sciences and Technology | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
dc.contributor.mitauthor | Zetsche, Bernd | |
dc.contributor.mitauthor | Gootenberg, Jonathan S | |
dc.contributor.mitauthor | Abudayyeh, Omar Osama | |
dc.contributor.mitauthor | Slaymaker, Ian | |
dc.contributor.mitauthor | Essletzbichler, Patrick | |
dc.contributor.mitauthor | Volz, Sara E. | |
dc.contributor.mitauthor | Joung, Julia | |
dc.contributor.mitauthor | Regev, Aviv | |
dc.contributor.mitauthor | Zhang, Feng | |
dc.relation.journal | Cell | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Zetsche, Bernd; Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Slaymaker, Ian M.; Makarova, Kira S.; Essletzbichler, Patrick; Volz, Sara E.; Joung, Julia; van der Oost, John; Regev, Aviv; Koonin, Eugene V.; Zhang, Feng | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7979-3220 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8794-2137 | |
dc.identifier.orcid | https://orcid.org/0000-0001-6656-5002 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8567-2049 | |
dc.identifier.orcid | https://orcid.org/0000-0003-2782-2509 | |
mit.license | PUBLISHER_CC | en_US |