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The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones

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The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones

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Author: Truttmann, Matthias C.; Cruz, Victor E.; Guo, Xuanzong; Engert, Christoph; Schwartz, Thomas U.; Ploegh, Hidde L.
Department: Massachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research; Massachusetts Institute of Technology. Computational and Systems Biology Program
Publisher: Public Library of Science
Date Issued: 2016-05
Abstract:
Protein AMPylation by Fic domain-containing proteins (Fic proteins) is an ancient and conserved post-translational modification of mostly unexplored significance. Here we characterize the Caenorhabditis elegans Fic protein FIC-1 in vitro and in vivo. FIC-1 is an AMPylase that localizes to the nuclear surface and modifies core histones H2 and H3 as well as heat shock protein 70 family members and translation elongation factors. The three-dimensional structure of FIC-1 is similar to that of its human ortholog, HYPE, with 38% sequence identity. We identify a link between FIC-1-mediated AMPylation and susceptibility to the pathogen Pseudomonas aeruginosa, establishing a connection between AMPylation and innate immunity in C. elegans.
URI: http://hdl.handle.net/1721.1/105785
ISSN: 1553-7404
Citation: Truttmann, Matthias C., Victor E. Cruz, Xuanzong Guo, Christoph Engert, Thomas U. Schwartz, and Hidde L. Ploegh. “The Caenorhabditis Elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.” Edited by Man-Wah Tan. PLOS Genetics 12, no. 5 (May 3, 2016): e1006023.
Version: Final published version
Terms of Use: Creative Commons Attribution 4.0 International License
Detailed Terms: http://creativecommons.org/licenses/by/4.0/
Published As: http://dx.doi.org/10.1371/journal.pgen.1006023
Journal: PLOS Genetics

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