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Lack of Evidence for PKM2 Protein Kinase Activity

Author(s)
Hosios, Aaron Marc; Fiske, Brian Prescott; Gui, Dan Yi; Vander Heiden, Matthew G.
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Abstract
The role of pyruvate kinase M2 (PKM2) in cell proliferation is controversial. A unique function of PKM2 proposed to be important for the proliferation of some cancer cells involves the direct activity of this enzyme as a protein kinase; however, a detailed biochemical characterization of this activity is lacking. Using [32P]-phosphoenolpyruvate (PEP) we examine the direct substrates of PKM2 using recombinant enzyme and in vitro systems where PKM2 is genetically deleted. Labeling of some protein species from [32P]-PEP can be observed; however, most were dependent on the presence of ADP, and none were dependent on the presence of PKM2. In addition, we also failed to observe PKM2-dependent transfer of phosphate from ATP directly to protein. These findings argue against a role for PKM2 as a protein kinase.
Date issued
2015-08
URI
http://hdl.handle.net/1721.1/105827
Department
Massachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal
Molecular Cell
Publisher
Elsevier
Citation
Hosios, Aaron M. et al. “Lack of Evidence for PKM2 Protein Kinase Activity.” Molecular Cell 59.5 (2015): 850–857.
Version: Author's final manuscript
ISSN
10972765

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