Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging
Author(s)
Husvogt, Lennart; Vardeh, Hilde; Faulkner-Jones, Beverly E.; Hornegger, Joachim; Connolly, James L.; Giacomelli, Michael; Fujimoto, James G; ... Show more Show less
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We derive a physically realistic model for the generation of virtual transillumination, white light microscopy images using epi-fluorescence measurements from thick, unsectioned tissue. We demonstrate this technique by generating virtual transillumination H&E images of unsectioned human breast tissue from epi-fluorescence multiphoton microscopy data. The virtual transillumination algorithm is shown to enable improved contrast and color accuracy compared with previous color mapping methods. Finally, we present an open source implementation of the algorithm in OpenGL, enabling real-time GPU-based generation of virtual transillumination microscopy images using conventional fluorescence microscopy systems.
Date issued
2016-08Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer ScienceJournal
PLOS ONE
Publisher
Public Library of Science
Citation
Giacomelli, Michael G. et al. “Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging.” Ed. Thomas Abraham. PLOS ONE 11.8 (2016): e0159337.
Version: Final published version
ISSN
1932-6203