In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light

Author(s)
Moore, Anna V; Ran, Chongzhao; Zhang, Zhaoda; Hooker, Jacob
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Abstract
Purpose The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET) radiotracer, 2-deoxy-2-[[superscript 18]F]fluoro-d-glucose ([superscript 18]FDG), could be used as an alternative light source for photoactivation. Procedures We utilized [superscript 18]FDG, which is a metabolic activity-based PET probe, as a source of light to photoactivate caged luciferin in a breast cancer animal model expressing luciferase. Results Bioluminescence produced from luciferin allowed for the real-time monitoring of Cherenkov radiation-promoted uncaging of the substrate. Conclusion The proposed method may provide a very important option for in vivo photoactivation, in particular for activation of photosensitizers for photodynamic therapy and eventually for combining radioisotope therapy and photodynamic therapy.
Date issued
2011-05
URI
http://hdl.handle.net/1721.1/106641
Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science; Martinos Imaging Center (McGovern Institute for Brain Research at MIT); Harvard University--MIT Division of Health Sciences and Technology; McGovern Institute for Brain Research at MIT
Journal
Molecular Imaging and Biology
Publisher
Springer-Verlag
Citation
Ran, Chongzhao et al. “In Vivo Photoactivation Without ‘Light’: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light.” Molecular Imaging and Biology 14.2 (2012): 156–162.
Version: Author's final manuscript
ISSN
1536-1632
1860-2002
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