In Vivo Photoactivation Without “Light”: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light
Author(s)Moore, Anna V; Ran, Chongzhao; Zhang, Zhaoda; Hooker, Jacob
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Purpose The poor tissue penetration of visible light has been a major barrier for optical imaging, photoactivatable conversions, and photodynamic therapy for in vivo targets with depths beyond 10 mm. In this report, as a proof-of-concept, we demonstrated that a positron emission tomography (PET) radiotracer, 2-deoxy-2-[[superscript 18]F]fluoro-d-glucose ([superscript 18]FDG), could be used as an alternative light source for photoactivation. Procedures We utilized [superscript 18]FDG, which is a metabolic activity-based PET probe, as a source of light to photoactivate caged luciferin in a breast cancer animal model expressing luciferase. Results Bioluminescence produced from luciferin allowed for the real-time monitoring of Cherenkov radiation-promoted uncaging of the substrate. Conclusion The proposed method may provide a very important option for in vivo photoactivation, in particular for activation of photosensitizers for photodynamic therapy and eventually for combining radioisotope therapy and photodynamic therapy.
DepartmentInstitute for Medical Engineering and Science; Martinos Imaging Center at MIT; Harvard University--MIT Division of Health Sciences and Technology; McGovern Institute for Brain Research at MIT
Molecular Imaging and Biology
Ran, Chongzhao et al. “In Vivo Photoactivation Without ‘Light’: Use of Cherenkov Radiation to Overcome the Penetration Limit of Light.” Molecular Imaging and Biology 14.2 (2012): 156–162.
Author's final manuscript