Designer nanoscale DNA assemblies programmed from the top down
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Bathe_Designer nanoscale DNA.pdf
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Author(s)
Zhang, K.
Zhang, F.
Yan, H.
Chiu, W.
Veneziano, Remi
Ratanalert, Sakul
Bathe, Mark
Date Issued
May 2016
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Veneziano, R. et al. “Designer Nanoscale DNA Assemblies Programmed from the Top down.” Science 352.6293 (2016): 1534–1534.
Version
Author's final manuscript
Abstract
Scaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach is limited by the need to forward-design specific Watson-Crick basepairing manually for any given target structure. Here, we report a general, top-down strategy to
design nearly arbitrary DNA architectures autonomously based only on target shape. Objects are represented as closed surfaces rendered as polyhedral networks of parallel DNA duplexes, which enables complete DNA scaffold routing with a spanning tree algorithm. The asymmetric polymerase chain reaction was applied to produce stable, monodisperse assemblies with custom scaffold length and sequence that are verified structurally in 3D to be high fidelity using single-particle cryo-electron microscopy. Their long-term stability in serum and low-salt buffer confirms their utility for biological as well as nonbiological applications.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.aaf4388
