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dc.contributor.authorvon Rohrscheidt, Julia
dc.contributor.authorPetrozziello, Elisabetta
dc.contributor.authorNedjic, Jelena
dc.contributor.authorFederle, Christine
dc.contributor.authorKrzyzak, Lena
dc.contributor.authorIshido, Satoshi
dc.contributor.authorSteinkasserer, Alexander
dc.contributor.authorKlein, Ludger
dc.contributor.authorPloegh, Hidde
dc.date.accessioned2017-02-23T20:17:54Z
dc.date.available2017-02-23T20:17:54Z
dc.date.issued2016-08
dc.date.submitted2016-02
dc.identifier.issn0022-1007
dc.identifier.issn1540-9538
dc.identifier.urihttp://hdl.handle.net/1721.1/107138
dc.description.abstractDeficiency of CD83 in thymic epithelial cells (TECs) dramatically impairs thymic CD4 T cell selection. CD83 can exert cell-intrinsic and –extrinsic functions through discrete protein domains, but it remains unclear how CD83’s capacity to operate through these alternative functional modules relates to its crucial role in TECs. In this study, using viral reconstitution of gene function in TECs, we found that CD83’s transmembrane domain is necessary and sufficient for thymic CD4 T cell selection. Moreover, a ubiquitination-resistant MHCII variant restored CD4 T cell selection in Cd83[superscript −/−] mice. Although during dendritic cell maturation CD83 is known to stabilize MHCII through opposing the ubiquitin ligase March1, regulation of March1 did not account for CD83’s TEC-intrinsic role. Instead, we provide evidence that MHCII in cortical TECs (cTECs) is targeted by March8, an E3 ligase of as yet unknown physiological substrate specificity. Ablating March8 in Cd83[superscript −/−] mice restored CD4 T cell development. Our results identify CD83-mediated MHCII stabilization through antagonism of March8 as a novel functional adaptation of cTECs for T cell selection. Furthermore, these findings suggest an intriguing division of labor between March1 and March8 in controlling inducible versus constitutive MHCII expression in hematopoietic antigen-presenting cells versus TECs.en_US
dc.language.isoen_US
dc.publisherRockefeller University Pressen_US
dc.relation.isversionofhttp://dx.doi.org/10.1084/jem.20160316en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceRockefeller University Pressen_US
dc.titleThymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83en_US
dc.typeArticleen_US
dc.identifier.citationvon Rohrscheidt, Julia et al. “Thymic CD4 T Cell Selection Requires Attenuation of March8-Mediated MHCII Turnover in Cortical Epithelial Cells through CD83.” The Journal of Experimental Medicine 213.9 (2016): 1685–1694.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorPloegh, Hidde
dc.relation.journalThe Journal of Experimental Medicineen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsvon Rohrscheidt, Julia; Petrozziello, Elisabetta; Nedjic, Jelena; Federle, Christine; Krzyzak, Lena; Ploegh, Hidde L.; Ishido, Satoshi; Steinkasserer, Alexander; Klein, Ludgeren_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-1090-6071
mit.licensePUBLISHER_CCen_US


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