Atomic structure of the Y complex of the nuclear pore
Author(s)Kelley, Kotaro; Knockenhauer, Kevin Edward; Kabachinski, Gregory L.; Schwartz, Thomas
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The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. Here we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.
available in PMC 2015 November 01
DepartmentMassachusetts Institute of Technology. Department of Biology
Nature Structural & Molecular Biology
Kelley, Kotaro, Kevin E Knockenhauer, Greg Kabachinski, and Thomas U Schwartz. “Atomic Structure of the Y Complex of the Nuclear Pore.” Nature Structural & Molecular Biology (March 30, 2015).
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