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dc.contributor.authorSantschi, Christian
dc.contributor.authorVon Moos, Nadia
dc.contributor.authorBowen, Paul
dc.contributor.authorSlaveykova, Vera I.
dc.contributor.authorMartin, Olivier J. F.
dc.contributor.authorKoman, Volodymyr
dc.date.accessioned2017-03-23T19:13:09Z
dc.date.available2017-03-23T19:13:09Z
dc.date.issued2017-03
dc.date.submitted2016-12
dc.identifier.issn1477-3155
dc.identifier.urihttp://hdl.handle.net/1721.1/107675
dc.description.abstractEngineered nanomaterials (ENMs) are key drivers for the development of highly sophisticated new technologies. As all new attainments, the rapidly increasing used of ENMs raise concerns about their safety for the environment and humans. There is growing evidence showing that if engineered nanomaterials are released into the environment, there is a possibility that they could cause harm to aquatic microorganisms. Among the divers effects triggering their toxicity the ability of ENMs to generate reactive oxygen species (ROS) capable of oxidizing biomolecules is currently considered a central mechanism of toxicity. Therefore, development of sensitive tools for quantification of the ROS generation and oxidative stress are highly sought. After briefly introducing ENMs-induced ROS generation and oxidative stress in the aquatic microorganisms (AMOs), this overview paper focuses on a new optical biosensor allowing sensitive and dynamic measurements of H[subscript 2]O[subscript 2] in real-time using multiscattering enhanced absorption spectroscopy. Its principle is based on sensitive absorption measurements of the heme protein cytochrome c whose absorption spectrum alters with the oxidation state of constituent ferrous Fe[superscript II] and ferric Fe[superscript III]. For biological applications cytochrome c was embedded in porous random media resulting in an extended optical path length through multiple scattering of light, which lowers the limit of detection to a few nM of H[subscript 2]O[subscript 2]. The sensor was also integrated in a microfluidic system containing micro-valves and sieves enabling more complex experimental conditions. To demonstrate its performance, abiotic absorption measurements of low concentrations of dye molecules and 10 nm gold particles were carried out achieving limits of detection in the low nM range. Other biologically relevant reactive oxygen species can be measured at sub-μM concentrations, which was shown for glucose and lactate through enzymatic reactions producing H[subscript 2]O[subscript 2]. In ecotoxicological investigations H[subscript 2]O[subscript 2]excreted by aquatic microorganisms exposed to various stressors were measured. Pro-oxidant effects of nano-TiO[subscript 2] and nano-CuO towards green alga Chlamydomonas reinhardtii were explored in various exposure media and under different light illuminations. Dynamics of Cd[superscript 2+] induced effects on photosynthetic activity, sensitisation and recovery of cells of C. reinhardtii was also studied.en_US
dc.description.sponsorshipSwiss National Science Foundation (Swiss National Research Program NRP 64 Project No. 406440-131280/1)en_US
dc.publisherBioMed Centralen_US
dc.relation.isversionofhttp://dx.doi.org/10.1186/s12951-017-0253-xen_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceBioMed Centralen_US
dc.titleNon-invasive continuous monitoring of pro-oxidant effects of engineered nanoparticles on aquatic microorganismsen_US
dc.typeArticleen_US
dc.identifier.citationSantschi, Christian et al. “Non-Invasive Continuous Monitoring of pro-Oxidant Effects of Engineered Nanoparticles on Aquatic Microorganisms.” Journal of Nanobiotechnology 15.1 (2017): n. pag.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorKoman, Volodymyr
dc.relation.journalJournal of Nanobiotechnologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dc.date.updated2017-03-08T04:40:04Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
dspace.orderedauthorsSantschi, Christian; Von Moos, Nadia; Koman, Volodymyr B.; Slaveykova, Vera I.; Bowen, Paul; Martin, Olivier J. F.en_US
dspace.embargo.termsNen_US
mit.licensePUBLISHER_CCen_US


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