Assembly and operation of the autopatcher for automated intracellular neural recording in vivo
Author(s)
Kodandaramaiah, Suhasa B; Holst, Gregory L; Singer, Annabelle C; Franzesi, Giovanni Talei; McKinnon, Michael L; Forest, Craig R; Wickersham, Ian R.; Boyden, Edward; ... Show more Show less
DownloadBoyden_Assembly and Operation of the Autopatcher.pdf (1.480Mb)
OPEN_ACCESS_POLICY
Open Access Policy
Creative Commons Attribution-Noncommercial-Share Alike
Terms of use
Metadata
Show full item recordAbstract
Whole-cell patch clamping in vivo is an important neuroscience technique that uniquely provides access to both suprathreshold spiking and subthreshold synaptic events of single neurons in the brain. This article describes how to set up and use the autopatcher, which is a robot for automatically obtaining high-yield and high-quality whole-cell patch clamp recordings in vivo. By following this protocol, a functional experimental rig for automated whole-cell patch clamping can be set up in 1 week. High-quality surgical preparation of mice takes ~1 h, and each autopatching experiment can be carried out over periods lasting several hours. Autopatching should enable in vivo intracellular investigations to be accessible by a substantial number of neuroscience laboratories, and it enables labs that are already doing in vivo patch clamping to scale up their efforts by reducing training time for new lab members and increasing experimental durations by handling mentally intensive tasks automatically.
Date issued
2016-03Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; Massachusetts Institute of Technology. Media Laboratory; McGovern Institute for Brain Research at MITJournal
Nature Protocols
Publisher
Nature Publishing Group
Citation
Kodandaramaiah, Suhasa B, Gregory L Holst, Ian R Wickersham, Annabelle C Singer, Giovanni Talei Franzesi, Michael L McKinnon, Craig R Forest, and Edward S Boyden. “Assembly and Operation of the Autopatcher for Automated Intracellular Neural Recording in Vivo.” Nature Protocols 11, no. 4 (March 3, 2016): 634–654.
Version: Author's final manuscript
ISSN
1754-2189
1750-2799