RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin
Author(s)
Engreitz, Jesse Michael; Lander, Eric Steven; Guttman, Mitchell
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RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing.
Date issued
2015Department
Institute for Medical Engineering and Science; Massachusetts Institute of Technology. Department of BiologyJournal
Nuclear Bodies and Noncoding RNAs
Publisher
Springer New York
Citation
Engreitz, Jesse, Lander, Eric S., and Guttman, Mitchell. “RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.” Nuclear Bodies and Noncoding RNAs (December 5, 2014): 183–197.
Version: Author's final manuscript
ISSN
1064-3745
1940-6029