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dc.contributor.authorChoi, Myunghwan
dc.contributor.authorBhayana, Brijesh
dc.contributor.authorZhang, Xueli
dc.contributor.authorRan, Chongzhao
dc.contributor.authorKwok, Sheldon J.J
dc.contributor.authorYun, Seok Hyun (Andy)
dc.date.accessioned2017-04-21T17:29:50Z
dc.date.available2017-04-21T17:29:50Z
dc.date.issued2016-03
dc.date.submitted2015-10
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/108348
dc.description.abstractThe advent of phototransformable fluorescent proteins has led to significant advances in optical imaging, including the unambiguous tracking of cells over large spatiotemporal scales. However, these proteins typically require activating light in the UV-blue spectrum, which limits their in vivo applicability due to poor light penetration and associated phototoxicity on cells and tissue. We report that cyanine-based, organic dyes can be efficiently photoconverted by nonlinear excitation at the near infrared (NIR) window. Photoconversion likely involves singlet-oxygen mediated photochemical cleavage, yielding blue-shifted fluorescent products. Using SYTO62, a biocompatible and cell-permeable dye, we demonstrate photoconversion in a variety of cell lines, including depth-resolved labeling of cells in 3D culture. Two-photon photoconversion of cyanine-based dyes offer several advantages over existing photoconvertible proteins, including use of minimally toxic NIR light, labeling without need for genetic intervention, rapid kinetics, remote subsurface targeting, and long persistence of photoconverted signal. These findings are expected to be useful for applications involving rapid labeling of cells deep in tissue.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (grant P01HL120839)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (grant P41EB015903)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (grant R01CA192878)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep23866en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNature Publishing Groupen_US
dc.titleTwo-photon excited photoconversion of cyanine-based dyesen_US
dc.typeArticleen_US
dc.identifier.citationKwok, Sheldon J. J. et al. “Two-Photon Excited Photoconversion of Cyanine-Based Dyes.” Scientific Reports 6 (2016): 23866. © 2016 Macmillan Publishers Limiteden_US
dc.contributor.departmentMassachusetts Institute of Technology. Institute for Medical Engineering & Scienceen_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.mitauthorKwok, Sheldon J.J
dc.contributor.mitauthorYun, Seok Hyun (Andy)
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsKwok, Sheldon J. J.; Choi, Myunghwan; Bhayana, Brijesh; Zhang, Xueli; Ran, Chongzhao; Yun, Seok-Hyunen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-5230-4669
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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