Show simple item record

dc.contributor.authorMason, Frank M
dc.contributor.authorXie, Shicong
dc.contributor.authorVasquez, Claudia G
dc.contributor.authorMartin, Adam C
dc.contributor.authorTworoger, Michael B.
dc.date.accessioned2017-05-02T18:15:11Z
dc.date.available2017-05-02T18:15:11Z
dc.date.issued2016-08
dc.date.submitted2016-03
dc.identifier.issn0021-9525
dc.identifier.issn1540-8140
dc.identifier.urihttp://hdl.handle.net/1721.1/108604
dc.description.abstractDuring morphogenesis, contraction of the actomyosin cytoskeleton within individual cells drives cell shape changes that fold tissues. Coordination of cytoskeletal contractility is mediated by regulating RhoA GTPase activity. Guanine nucleotide exchange factors (GEFs) activate and GTPase-activating proteins (GAPs) inhibit RhoA activity. Most studies of tissue folding, including apical constriction, have focused on how RhoA is activated by GEFs to promote cell contractility, with little investigation as to how GAPs may be important. Here, we identify a critical role for a RhoA GAP, Cumberland GAP (C-GAP), which coordinates with a RhoA GEF, RhoGEF2, to organize spatiotemporal contractility during Drosophila melanogaster apical constriction. C-GAP spatially restricts RhoA pathway activity to a central position in the apical cortex. RhoGEF2 pulses precede myosin, and C-GAP is required for pulsation, suggesting that contractile pulses result from RhoA activity cycling. Finally, C-GAP expression level influences the transition from reversible to irreversible cell shape change, which defines the onset of tissue shape change. Our data demonstrate that RhoA activity cycling and modulating the ratio of RhoGEF2 to C-GAP are required for tissue folding.en_US
dc.description.sponsorshipAmerican Cancer Society (125792-RSG-14-039-01-CSM)en_US
dc.language.isoen_US
dc.publisherRockefeller University Press, Theen_US
dc.relation.isversionofhttp://dx.doi.org/10.1083/jcb.201603077en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0 Unported licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceRockefeller University Pressen_US
dc.titleRhoA GTPase inhibition organizes contraction during epithelial morphogenesisen_US
dc.typeArticleen_US
dc.identifier.citationMason, Frank M.; Xie, Shicong; Vasquez, Claudia G.; Tworoger, Michael and Martin, Adam C. “RhoA GTPase Inhibition Organizes Contraction During Epithelial Morphogenesis.” The Journal of Cell Biology 214, no. 5 (August 2016): 603–617. © 2016 Mason et al.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Computational and Systems Biology Programen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorMason, Frank M
dc.contributor.mitauthorXie, Shicong
dc.contributor.mitauthorVasquez, Claudia G
dc.contributor.mitauthorTworoger, Michael B
dc.contributor.mitauthorMartin, Adam C
dc.relation.journalJournal of Cell Biologyen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsMason, Frank M.; Xie, Shicong; Vasquez, Claudia G.; Tworoger, Michael; Martin, Adam C.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1338-494X
dc.identifier.orcidhttps://orcid.org/0000-0002-3283-3248
dc.identifier.orcidhttps://orcid.org/0000-0002-8987-7508
dc.identifier.orcidhttps://orcid.org/0000-0001-8060-2607
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record