dc.contributor.author | Baratta, Michael V | |
dc.contributor.author | Fropf, Robin | |
dc.contributor.author | Hemberg, Martin | |
dc.contributor.author | Yin, Jerry CP | |
dc.contributor.author | Maier, Steven F | |
dc.contributor.author | Sorensen, Andreas | |
dc.contributor.author | Cooper, Yonatan Ariel | |
dc.contributor.author | Weng, Feng-Ju | |
dc.contributor.author | Zhang, Yuxiang | |
dc.contributor.author | Ramamoorthi, Kartik | |
dc.contributor.author | LaVerriere, Emily Kathryn | |
dc.contributor.author | Xue, Jian | |
dc.contributor.author | Young, Andrew | |
dc.contributor.author | Schneider, Colleen | |
dc.contributor.author | Goetzsche, Casper | |
dc.contributor.author | Lin, Yingxi | |
dc.date.accessioned | 2017-05-09T13:50:14Z | |
dc.date.available | 2017-05-09T13:50:14Z | |
dc.date.issued | 2016-09 | |
dc.date.submitted | 2015-12 | |
dc.identifier.issn | 2050-084X | |
dc.identifier.uri | http://hdl.handle.net/1721.1/108765 | |
dc.description.abstract | Understanding how the brain captures transient experience and converts it into long lasting changes in neural circuits requires the identification and investigation of the specific ensembles of neurons that are responsible for the encoding of each experience. We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized synthetic activity-regulated promoter that is strongly induced by neuronal activity and a modified Tet-Off system that achieves improved temporal control. Due to its compact design, RAM can be packaged into a single adeno-associated virus (AAV), providing great versatility and ease of use, including application to mice, rats, flies, and potentially many other species. Cre-dependent RAM, CRAM, allows for the study of active ensembles of a specific cell type and anatomical connectivity, further expanding the RAM system’s versatility. | en_US |
dc.description.sponsorship | Swedish Brain Foundation (Research Fellowship) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.). Ruth L. Kirschstein National Research Service Award (Predoctoral Fellowship) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grants MH091220) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant NS088421) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant MH106817) | en_US |
dc.description.sponsorship | MIT Simons Initiatives on Autism and the Brain (Postdoctoral Fellowship) | en_US |
dc.description.sponsorship | John Merck Fund (Scholars Program) | en_US |
dc.language.iso | en_US | |
dc.publisher | eLife Sciences Publications, Ltd. | en_US |
dc.relation.isversionof | http://dx.doi.org/10.7554/eLife.13918 | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | eLife | en_US |
dc.title | A robust activity marking system for exploring active neuronal ensembles | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Sørensen, Andreas T et al. “A Robust Activity Marking System for Exploring Active Neuronal Ensembles.” eLife 5 (2016): n. pag. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
dc.contributor.mitauthor | Sorensen, Andreas | |
dc.contributor.mitauthor | Cooper, Yonatan Ariel | |
dc.contributor.mitauthor | Weng, Feng-Ju | |
dc.contributor.mitauthor | Zhang, Yuxiang | |
dc.contributor.mitauthor | Ramamoorthi, Kartik | |
dc.contributor.mitauthor | LaVerriere, Emily Kathryn | |
dc.contributor.mitauthor | Xue, Jian | |
dc.contributor.mitauthor | Young, Andrew | |
dc.contributor.mitauthor | Schneider, Colleen | |
dc.contributor.mitauthor | Goetzsche, Casper | |
dc.contributor.mitauthor | Lin, Yingxi | |
dc.relation.journal | eLife | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Sørensen, Andreas T; Cooper, Yonatan A; Baratta, Michael V; Weng, Feng-Ju; Zhang, Yuxiang; Ramamoorthi, Kartik; Fropf, Robin; LaVerriere, Emily; Xue, Jian; Young, Andrew; Schneider, Colleen; Gøtzsche, Casper René; Hemberg, Martin; Yin, Jerry CP; Maier, Steven F; Lin, Yingxi | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0002-7002-1275 | |
mit.license | PUBLISHER_CC | en_US |