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dc.contributor.authorGreen, Alexander A.
dc.contributor.authorKim, Jongmin
dc.contributor.authorMa, Duo
dc.contributor.authorSilver, Pamela A.
dc.contributor.authorYin, Peng
dc.contributor.authorCollins, James J.
dc.date.accessioned2017-05-19T13:47:04Z
dc.date.available2017-05-19T13:47:04Z
dc.date.issued2016-09
dc.date.submitted2016-09
dc.identifier.issn978-1-4503-4061-8
dc.identifier.urihttp://hdl.handle.net/1721.1/109202
dc.description.abstractSynthetic biology aims to create functional devices, systems, and organisms with novel and useful functions taking advantage of engineering principles applied to biology. Despite great progress over the last decade, an underlying problem in synthetic biology remains the limited number of high-performance, modular, composable parts. A potential route to solve parts bottleneck problem in synthetic biology utilizes the programmability of nucleic acids inspired by molecular programming approaches that have demonstrated complex biomolecular circuits evaluating logic expressions in test tubes.Using a library of de-novo-designed toehold switches with orthogonality and modular composability, we demonstrate how toehold switches can be incorporated into decision-making RNA networks termed ribocomputing devices to rapidly evaluate complex logic in living cells. We have successfully demonstrated a 4-input AND gate, a 6-input OR gate, and a 12-input expression in disjunctive normal form in E. coli. The compact encoding of ribocomputing system using a library of modular parts is amenable to aggressive scale-up towards complex control of in vivo circuitry towards autonomous behaviors and biomedical applications.en_US
dc.language.isoen_US
dc.publisherAssociation for Computing Machinery (ACM)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1145/2967446.2970373en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceProf. Collins via Howard Silveren_US
dc.titleRibocomputing devices for sophisticated in vivo logic computationen_US
dc.typeArticleen_US
dc.identifier.citationGreen, Alexander A.; Kim, Jongmin; Ma, Duo; Silver, Pamela A.; Collins, James J. and Yin, Peng. “Ribocomputing Devices for Sophisticated in Vivo Logic Computation.” NANOCOM’16, Proceedings of the 3rd ACM International Conference on Nanoscale Computing and Communication, September 28-30 2016, New York, New York, Association for Computing Machinery (ACM), September 2016en_US
dc.contributor.departmentInstitute for Medical Engineering and Scienceen_US
dc.contributor.approverCollins, Jamesen_US
dc.contributor.mitauthorCollins, James J.
dc.relation.journalNANOCOM '16, Proceedings of the 3rd ACM International Conference on Nanoscale Computing and Communicationen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/ConferencePaperen_US
eprint.statushttp://purl.org/eprint/status/NonPeerRevieweden_US
dspace.orderedauthorsGreen, Alexander A.; Kim, Jongmin; Ma, Duo; Silver, Pamela A.; Collins, James J.; Yin, Pengen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-5560-8246
mit.licenseOPEN_ACCESS_POLICYen_US


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