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dc.contributor.authorHu, Peng
dc.contributor.authorChakraborty, Sagar
dc.contributor.authorKumar, Amit
dc.contributor.authorWoolston, Benjamin Michael
dc.contributor.authorLiu, Hongjuan
dc.contributor.authorEmerson, David Frederic
dc.contributor.authorStephanopoulos, Gregory
dc.date.accessioned2017-05-22T19:55:33Z
dc.date.available2017-05-22T19:55:33Z
dc.date.issued2016-01
dc.date.submitted2015-08
dc.identifier.issn0027-8424
dc.identifier.issn1091-6490
dc.identifier.urihttp://hdl.handle.net/1721.1/109271
dc.description.abstractIn the quest for inexpensive feedstocks for the cost-effective production of liquid fuels, we have examined gaseous substrates that could be made available at low cost and sufficiently large scale for industrial fuel production. Here we introduce a new bioconversion scheme that effectively converts syngas, generated from gasification of coal, natural gas, or biomass, into lipids that can be used for biodiesel production. We present an integrated conversion method comprising a two-stage system. In the first stage, an anaerobic bioreactor converts mixtures of gases of CO₂ and CO or H₂ to acetic acid, using the anaerobic acetogen Moorella thermoacetica. The acetic acid product is fed as a substrate to a second bioreactor, where it is converted aerobically into lipids by an engineered oleaginous yeast, Yarrowia lipolytica. We first describe the process carried out in each reactor and then present an integrated system that produces microbial oil, using synthesis gas as input. The integrated continuous bench-scale reactor system produced 18 g/L of C16-C18 triacylglycerides directly from synthesis gas, with an overall productivity of 0.19 g⋅L⁻¹⋅h⁻¹ and a lipid content of 36%. Although suboptimal relative to the performance of the individual reactor components, the presented integrated system demonstrates the feasibility of substantial net fixation of carbon dioxide and conversion of gaseous feedstocks to lipids for biodiesel production. The system can be further optimized to approach the performance of its individual units so that it can be used for the economical conversion of waste gases from steel mills to valuable liquid fuels for transportation.en_US
dc.description.sponsorshipUnited States. Advanced Research Projects Agency-Energy (DE-AR0000059)en_US
dc.description.sponsorshipUnited States. Department of Energy (DE-SC0008744)en_US
dc.language.isoen_US
dc.publisherNational Academy of Sciences (U.S.)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1073/pnas.1516867113en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePNASen_US
dc.titleIntegrated bioprocess for conversion of gaseous substrates to liquidsen_US
dc.typeArticleen_US
dc.identifier.citationHu, Peng; Chakraborty, Sagar; Kumar, Amit; Woolston, Benjamin; Liu, Hongjuan; Emerson, David and Stephanopoulos, Gregory “Integrated Bioprocess for Conversion of Gaseous Substrates to Liquids.” Proceedings of the National Academy of Sciences 113, no. 14 (March 2016): 3773–3778 © 2016 National Academy of Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorHu, Peng
dc.contributor.mitauthorChakraborty, Sagar
dc.contributor.mitauthorKumar, Amit
dc.contributor.mitauthorWoolston, Benjamin Michael
dc.contributor.mitauthorLiu, Hongjuan
dc.contributor.mitauthorEmerson, David Frederic
dc.contributor.mitauthorStephanopoulos, Gregory
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHu, Peng; Chakraborty, Sagar; Kumar, Amit; Woolston, Benjamin; Liu, Hongjuan; Emerson, David; Stephanopoulos, Gregoryen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-6570-2236
dc.identifier.orcidhttps://orcid.org/0000-0003-4948-9962
dc.identifier.orcidhttps://orcid.org/0000-0001-6909-4568
mit.licensePUBLISHER_POLICYen_US


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