dc.contributor.author | Zhang, Chi | |
dc.contributor.author | Welborn, Matthew Gregory | |
dc.contributor.author | Zhu, Tianyu | |
dc.contributor.author | Yang, Nicole Jie Yeon | |
dc.contributor.author | Santos, Michael | |
dc.contributor.author | Van Voorhis, Troy | |
dc.contributor.author | Pentelute, Bradley L. | |
dc.date.accessioned | 2017-05-26T17:52:46Z | |
dc.date.available | 2017-05-26T17:52:46Z | |
dc.date.issued | 2015-12 | |
dc.date.submitted | 2014-12 | |
dc.identifier.issn | 1755-4330 | |
dc.identifier.issn | 1755-4349 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/109381 | |
dc.description.abstract | Site-selective functionalization of complex molecules is one of the most significant challenges in chemistry. Typically, protecting groups or catalysts must be used to enable the selective modification of one site among many that are similarly reactive, and general strategies that selectively tune the local chemical environment around a target site are rare. Here, we show a four-amino-acid sequence (Phe-Cys-Pro-Phe), which we call the ‘π-clamp’, that tunes the reactivity of its cysteine thiol for site-selective conjugation with perfluoroaromatic reagents. We use the π-clamp to selectively modify one cysteine site in proteins containing multiple endogenous cysteine residues. These examples include antibodies and cysteine-based enzymes that would be difficult to modify selectively using standard cysteine-based methods. Antibodies modified using the π-clamp retained binding affinity to their targets, enabling the synthesis of site-specific antibody–drug conjugates for selective killing of HER2-positive breast cancer cells. The π-clamp is an unexpected approach to mediate site-selective chemistry and provides new avenues to modify biomolecules for research and therapeutics. | en_US |
dc.description.sponsorship | Massachusetts Institute of Technology (Start-up Funds) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH R01GM110535) | en_US |
dc.description.sponsorship | Sontag Foundation (Distinguished Scientist Award) | en_US |
dc.description.sponsorship | Massachusetts Institute of Technology. Department of Chemistry (George Buchi Research Fellowship) | en_US |
dc.description.sponsorship | David H. Koch Institute for Integrative Cancer Research at MIT (Graduate Fellowship) | en_US |
dc.description.sponsorship | Bristol-Myers Squibb Company (Graduate Fellowship in Synthetic Organic Chemistry) | en_US |
dc.description.sponsorship | National Science Foundation (U.S.) (Graduate Research Fellow) | en_US |
dc.language.iso | en_US | |
dc.publisher | Springer Nature | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/nchem.2413 | en_US |
dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
dc.source | PMC | en_US |
dc.title | π-Clamp-mediated cysteine conjugation | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Zhang, Chi, Matthew Welborn, Tianyu Zhu, Nicole J. Yang, Michael S. Santos, Troy Van Voorhis, and Bradley L. Pentelute. "π-Clamp-mediated cysteine conjugation." Nature Chemistry 8 (2016), pp.120-128. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
dc.contributor.mitauthor | Zhang, Chi | |
dc.contributor.mitauthor | Welborn, Matthew Gregory | |
dc.contributor.mitauthor | Zhu, Tianyu | |
dc.contributor.mitauthor | Yang, Nicole Jie Yeon | |
dc.contributor.mitauthor | Santos, Michael | |
dc.contributor.mitauthor | Van Voorhis, Troy | |
dc.contributor.mitauthor | Pentelute, Bradley L. | |
dc.relation.journal | Nature Chemistry | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Zhang, Chi; Welborn, Matthew; Zhu, Tianyu; Yang, Nicole J.; Santos, Michael S.; Van Voorhis, Troy; Pentelute, Bradley L. | en_US |
dspace.embargo.terms | N | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-9519-7456 | |
dc.identifier.orcid | https://orcid.org/0000-0001-8659-6535 | |
dc.identifier.orcid | https://orcid.org/0000-0003-2061-3237 | |
dc.identifier.orcid | https://orcid.org/0000-0002-0882-7761 | |
dc.identifier.orcid | https://orcid.org/0000-0002-6229-9592 | |
dc.identifier.orcid | https://orcid.org/0000-0001-7111-0176 | |
mit.license | OPEN_ACCESS_POLICY | en_US |