Show simple item record

dc.contributor.authorWang, Yuguang
dc.contributor.authorLyu, Peijun
dc.contributor.authorHuang, Yingying
dc.contributor.authorWang, Yong
dc.contributor.authorHamblin, Michael R
dc.date.accessioned2017-05-31T17:39:50Z
dc.date.available2017-05-31T17:39:50Z
dc.date.issued2016-09
dc.date.submitted2016-04
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/1721.1/109470
dc.description.abstractHuman adipose-derived stem cells (hASCs) have the potential to differentiate into several different cell types including osteoblasts. Photobiomodulation (PBM) or low level laser therapy (LLLT) using red or near-infrared wavelengths has been reported to have effects on both proliferation and osteogenic differentiation of stem cells. We examined the effects of delivering four different wavelengths (420 nm, 540 nm, 660 nm, 810 nm) at the same dose (3 J/cm2) five times (every two days) on hASCs cultured in osteogenic medium over three weeks. We measured expression of the following transcription factors by RT-PCR: RUNX2, osterix, and the osteoblast protein, osteocalcin. The 420 nm and 540 nm wavelengths were more effective in stimulating osteoblast differentiation compared to 660 nm and 810 nm. Intracellular calcium was higher after 420 nm and 540 nm, and could be inhibited by capsazepine and SKF96365, which also inhibited osteogenic differentiation. We hypothesize that activation of light-gated calcium ion channels by blue and green light could explain our results.en_US
dc.description.sponsorshipPeking University. School of Stomatology (grant PKUSS20140208)en_US
dc.description.sponsorshipChina. Ministry of Education (grant 113002A)en_US
dc.description.sponsorshipUnited States. National Institutes of Health (grant AI050875)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/srep33719en_US
dc.rightsCreative Commons Attribution 4.0 International Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceNatureen_US
dc.titlePhotobiomodulation (blue and green light) encourages osteoblastic-differentiation of human adipose-derived stem cells: role of intracellular calcium and light-gated ion channelsen_US
dc.typeArticleen_US
dc.identifier.citationWang, Yuguang, Ying-Ying Huang, Yong Wang, Peijun Lyu, and Michael R. Hamblin. “Photobiomodulation (blue and Green Light) Encourages Osteoblastic-Differentiation of Human Adipose-Derived Stem Cells: Role of Intracellular Calcium and Light-Gated Ion Channels.” Scientific Reports 6, no. 1 (September 21, 2016).en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.mitauthorHuang, Yingying
dc.contributor.mitauthorWang, Yong
dc.contributor.mitauthorHamblin, Michael R
dc.relation.journalScientific Reportsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsWang, Yuguang; Huang, Ying-Ying; Wang, Yong; Lyu, Peijun; Hamblin, Michael R.en_US
dspace.embargo.termsNen_US
mit.licensePUBLISHER_CCen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record