Show simple item record

dc.contributor.authorReece, Amy E.
dc.contributor.authorOakey, John
dc.contributor.authorKaastrup, Kaja
dc.contributor.authorSikes Johnson, Hadley
dc.date.accessioned2017-05-31T18:27:42Z
dc.date.available2017-05-31T18:27:42Z
dc.date.issued2015
dc.date.submitted2015-06
dc.identifier.issn2046-2069
dc.identifier.urihttp://hdl.handle.net/1721.1/109471
dc.description.abstractMicrofluidic inertial focusing reliably and passively aligns small particles and cells through a combination of competing inertial fluid forces. The equilibrium behavior of inertially focused particles in straight channels has been extensively characterized and has been shown to be a strong function of channel size, geometry and particle size. We demonstrate that channels of varying geometry may be combined to produce a staged device capable of high throughput particle and cell concentration and efficient single pass complex fluid enrichment. Straight and asymmetrically curved microchannels were combined in series to accelerate focusing dynamics and improve concentration efficiency. We have investigated single and multiple pass concentration efficiency and results indicate that these devices are appropriate for routine cell handling operations, including buffer exchange. We demonstrate the utility of these devices by performing a ubiquitous fluorescence staining assay on-chip while sacrificing very little sample or processing time relative to centrifugation. Staged concentration is particularly desirable for point of care settings in which more conventional instrumentation is impractical or cost-prohibitive.en_US
dc.description.sponsorshipUnited States. Department of Defense (Congressionally Directed Medical Research Program, Prostate Cancer Research Program Award number W81XWH-13-1-0272)en_US
dc.description.sponsorshipUniversity of Wyoming. IDeA Networks of Biomedical Research Excellence (program P20RR016474)en_US
dc.description.sponsorshipUniversity of Wyoming. IDeA Networks of Biomedical Research Excellence (program P20GM103432)en_US
dc.description.sponsorshipUnited States. Department of Defense (Congressionally Directed Medical Research Program, Prostate Cancer Research Program Award number W81XWH-13-1-0273)en_US
dc.description.sponsorshipUnited States. National Aeronautics and Space Administration (Wyoming NASA Space Grant Consortium (NASA Grant #NNX10A095H))en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Wyoming Experimental Program to Stimulate Competitive Research (Grant EPS-0447681))en_US
dc.language.isoen_US
dc.publisherRoyal Society of Chemistry (RSC)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1039/c5ra10634fen_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleStaged inertial microfluidic focusing for complex fluid enrichmenten_US
dc.typeArticleen_US
dc.identifier.citationReece, Amy E., Kaja Kaastrup, Hadley D. Sikes, and John Oakey. “Staged Inertial Microfluidic Focusing for Complex Fluid Enrichment.” RSC Adv. 5, no. 66 (2015): 53857–53864.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.mitauthorKaastrup, Kaja
dc.contributor.mitauthorSikes Johnson, Hadley
dc.relation.journalRSC Advancesen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsReece, Amy E.; Kaastrup, Kaja; Sikes, Hadley D.; Oakey, Johnen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-1720-0183
dc.identifier.orcidhttps://orcid.org/0000-0002-7096-138X
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record