In vivo label-free measurement of lymph flow velocity and volumetric flow rates using Doppler optical coherence tomography
Author(s)Blatter, Cedric; Meijer, Eelco F. J.; Jones, Dennis; Nam, Ahhyun; Padera, Timothy P; Bouma, Brett E; Vakoc, Benjamin; ... Show more Show less
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Direct in vivo imaging of lymph flow is key to understanding lymphatic system function in normal and disease states. Optical microscopy techniques provide the resolution required for these measurements, but existing optical techniques for measuring lymph flow require complex protocols and provide limited temporal resolution. Here, we describe a Doppler optical coherence tomography platform that allows direct, label-free quantification of lymph velocity and volumetric flow rates. We overcome the challenge of very low scattering by employing a Doppler algorithm that operates on low signal-to-noise measurements. We show that this technique can measure lymph velocity at sufficiently high temporal resolution to resolve the dynamic pulsatile flow in collecting lymphatic vessels.
DepartmentInstitute for Medical Engineering and Science; Massachusetts Institute of Technology. Department of Mechanical Engineering
Nature Publishing Group
Blatter, Cedric; Meijer, Eelco F. J.; Nam, Ahhyun S.; Jones, Dennis; Bouma, Brett E.; Padera, Timothy P. and Vakoc, Benjamin J. “In Vivo Label-Free Measurement of Lymph Flow Velocity and Volumetric Flow Rates Using Doppler Optical Coherence Tomography.” Scientific Reports 6 (July 2016): 29035 © 2017 Macmillan Publishers Limited, part of Springer Nature
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