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dc.contributor.authorUgolini, Giovanni Stefano
dc.contributor.authorPavesi, Andrea
dc.contributor.authorRasponi, Marco
dc.contributor.authorFiore, Gianfranco Beniamino
dc.contributor.authorSoncini, Monica
dc.contributor.authorKamm, Roger Dale
dc.date.accessioned2017-06-15T14:33:00Z
dc.date.available2017-06-15T14:33:00Z
dc.date.issued2017-03
dc.date.submitted2016-11
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/1721.1/109885
dc.description.abstractUpon cardiac pathological conditions such as ischemia, microenvironmental changes instruct a series of cellular responses that trigger cardiac fibroblasts-mediated tissue adaptation and inflammation. A comprehensive model of how early environmental changes may induce cardiac fibroblasts (CF) pathological responses is far from being elucidated, partly due to the lack of approaches involving complex and simultaneous environmental stimulation. Here, we provide a first analysis of human primary CF behavior by means of a multi-stimulus microdevice for combined application of cyclic mechanical strain and controlled oxygen tension. Our findings elucidate differential human CFs responses to different combinations of the above stimuli. Individual stimuli cause proliferative effects (PHH3+ mitotic cells, YAP translocation, PDGF secretion) or increase collagen presence. Interestingly, only the combination of hypoxia and a simulated loss of contractility (2% strain) is able to additionally induce increased CF release of inflammatory and pro-fibrotic cytokines and matrix metalloproteinases.en_US
dc.language.isoen_US
dc.publishereLife Sciences Publications, Ltd.en_US
dc.relation.isversionofhttp://dx.doi.org/10.7554/eLife.22847en_US
dc.rightsCreative Commons Attribution 4.0 International Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourceeLifeen_US
dc.titleHuman cardiac fibroblasts adaptive responses to controlled combined mechanical strain and oxygen changes in vitroen_US
dc.typeArticleen_US
dc.identifier.citationUgolini, Giovanni Stefano; Pavesi, Andrea; Rasponi, Marco; Fiore, Gianfranco Beniamino; Kamm, Roger and Soncini, Monica. “Human Cardiac Fibroblasts Adaptive Responses to Controlled Combined Mechanical Strain and Oxygen Changes in Vitro.” eLife 6 (March 2017): e22847 © 2017 Ugolini et alen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.mitauthorKamm, Roger Dale
dc.relation.journaleLifeen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsUgolini, Giovanni Stefano; Pavesi, Andrea; Rasponi, Marco; Fiore, Gianfranco Beniamino; Kamm, Roger; Soncini, Monicaen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7232-304X
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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