Evaluating anesthetic protocols for functional blood flow imaging in the rat eye
Author(s)
Choi, WooJhon; Boas, David A.; Clermont, Allen C.; Feener, Edward P.; Moult, Eric Michael; Baumann, Bernhard; Fujimoto, James G; ... Show more Show less
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The purpose of this study is to evaluate the suitability of five different anesthetic protocols (isoflurane, isoflurane–xylazine, pentobarbital, ketamine–xylazine, and ketamine–xylazine–vecuronium) for functional blood flow imaging in the rat eye. Total retinal blood flow was measured at a series of time points using an ultrahigh-speed Doppler OCT system. Additionally, each anesthetic protocol was qualitatively evaluated according to the following criteria: (1) time-stability of blood flow, (2) overall rate of blood flow, (3) ocular immobilization, and (4) simplicity. We observed that different anesthetic protocols produced markedly different blood flows. Different anesthetic protocols also varied with respect to the four evaluated criteria. These findings suggest that the choice of anesthetic protocol should be carefully considered when designing and interpreting functional blood flow studies in the rat eye.
Date issued
2017-01Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Research Laboratory of ElectronicsJournal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Moult, Eric M.; Choi, WooJhon; Boas, David A.; Baumann, Bernhard; Clermont, Allen C.; Feener, Edward P. and Fujimoto, James G. “Evaluating Anesthetic Protocols for Functional Blood Flow Imaging in the Rat Eye.” Journal of Biomedical Optics 22, no. 1 (January 2017): 016005 © 2016 The Authors
Version: Final published version
ISSN
1083-3668
1560-2281