A novel role for transcription-coupled nucleotide excision repair for the in vivo repair of 3, N[superscript4]-ethenocytosine
Author(s)Iyama, Teruaki; Chaim, Isaac Alexander; Gardner, Alycia M.; Wu, Jie; Wilson, David M.; Samson, Leona D; ... Show more Show less
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Etheno (ε) DNA base adducts are highly mutagenic lesions produced endogenously via reactions with lipid peroxidation (LPO) products. Cancer-promoting conditions, such as inflammation, can induce persistent oxidative stress and increased LPO, resulting in the accumulation of ε-adducts in different tissues. Using a recently described fluorescence multiplexed host cell reactivation assay, we show that a plasmid reporter bearing a site-specific 3,N4-ethenocytosine (εC) causes transcriptional blockage. Notably, this blockage is exacerbated in Cockayne Syndrome and xeroderma pigmentosum patient-derived lymphoblastoid and fibroblast cells. Parallel RNA-Seq expression analysis of the plasmid reporter identifies novel transcriptional mutagenesis properties of εC. Our studies reveal that beyond the known pathways, such as base excision repair, the process of transcription-coupled nucleotide excision repair plays a role in the removal of εC from the genome, and thus in the protection of cells and tissues from collateral damage induced by inflammatory responses.
DepartmentMassachusetts Institute of Technology. Center for Environmental Health Sciences; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology
Nucleic Acids Research
Oxford University Press
Chaim, Isaac A., Alycia Gardner, Jie Wu, Teruaki Iyama, David M. Wilson, and Leona D. Samson. “A Novel Role for Transcription-Coupled Nucleotide Excision Repair for the in Vivo repair of 3,N[superscript 4]-Ethenocytosine.” Nucleic Acids Research (January 23, 2017): gkx015.
Final published version