High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry
Author(s)Bekdemir, Ahmet; Yang, Yu-Sang Sabrina; Atukorale, Prabhani Upeka; Moynihan, Kelly Dare; Rakhra, Kavya; Tang, Li; Stellacci, Francesco; Irvine, Darrell J; ... Show more Show less
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Inorganic nanoparticles (NPs) are studied as drug carriers, radiosensitizers and imaging agents, and characterizing nanoparticle biodistribution is essential for evaluating their efficacy and safety. Tracking NPs at the single-cell level with current technologies is complicated by the lack of reliable methods to stably label particles over extended durations in vivo. Here we demonstrate that mass cytometry by time-of-flight provides a label-free approach for inorganic nanoparticle quantitation in cells. Furthermore, mass cytometry can enumerate AuNPs with a lower detection limit of ∼10 AuNPs (3 nm core size) in a single cell with tandem multiparameter cellular phenotyping. Using the cellular distribution insights, we selected an amphiphilic surface ligand-coated AuNP that targeted myeloid dendritic cells in lymph nodes as a peptide antigen carrier, substantially increasing the efficacy of a model vaccine in a B16-OVA melanoma mouse model. This technology provides a powerful new level of insight into nanoparticle fate in vivo.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Materials Science and Engineering
Nature Publishing Group
Yang, Yu-Sang Sabrina, Prabhani U. Atukorale, Kelly D. Moynihan, Ahmet Bekdemir, Kavya Rakhra, Li Tang, Francesco Stellacci, and Darrell J. Irvine. “High-Throughput Quantitation of Inorganic Nanoparticle Biodistribution at the Single-Cell Level Using Mass Cytometry.” Nature Communications 8 (January 17, 2017): 14069.
Final published version