MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus

Author(s)
Shlomai, Amir; Michailidis, Eleftherios; Bhatta, Ankit; Rice, Charles M.; Ramanan, Vyas; Cox, David Benjamin Turitz; Scott, David Arthur; Zhang, Feng; Bhatia, Sangeeta N; Schwartz, Robert E.; ... Show more Show less
Thumbnail
DownloadZhang_CRISPR.pdf (904.4Kb)
PUBLISHER_CC

Publisher with Creative Commons License

Creative Commons Attribution

Terms of use
Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/
Metadata
Show full item record
Abstract
Chronic hepatitis B virus (HBV) infection is prevalent, deadly, and seldom cured due to the persistence of viral episomal DNA (cccDNA) in infected cells. Newly developed genome engineering tools may offer the ability to directly cleave viral DNA, thereby promoting viral clearance. Here, we show that the CRISPR/Cas9 system can specifically target and cleave conserved regions in the HBV genome, resulting in robust suppression of viral gene expression and replication. Upon sustained expression of Cas9 and appropriately chosen guide RNAs, we demonstrate cleavage of cccDNA by Cas9 and a dramatic reduction in both cccDNA and other parameters of viral gene expression and replication. Thus, we show that directly targeting viral episomal DNA is a novel therapeutic approach to control the virus and possibly cure patients.
Date issued
2015-06
URI
http://hdl.handle.net/1721.1/110344
Department
Broad Institute of MIT and Harvard; Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT; Koch Institute for Integrative Cancer Research at MIT
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Ramanan, Vyas; Shlomai, Amir; Cox, David B.T.; Schwartz, Robert E.; Michailidis, Eleftherios; Bhatta, Ankit et al. "CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus." Scientific Reports 5 (June 2015): 1083 © 2015 Macmillan Publishers Limited, part of Springer Nature
Version: Final published version
ISSN
2045-2322

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.