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dc.contributor.authorMorrison, Michael James
dc.contributor.authorImperiali, Barbara
dc.date.accessioned2017-06-30T18:59:48Z
dc.date.available2017-06-30T18:59:48Z
dc.date.issued2013-08
dc.date.submitted2013-05
dc.identifier.issn0003-9861
dc.identifier.issn1096-0384
dc.identifier.urihttp://hdl.handle.net/1721.1/110400
dc.description.abstractThe Gram-negative, opportunistic pathogen Acinetobacter baumannii has recently captured headlines due to its ability to circumvent current antibiotic therapies. Herein we show that the multi-drug resistant (MDR) AYE strain of A. baumannii contains a gene locus that encodes three enzymes responsible for the biosynthesis of the highly-modified bacterial nucleotide sugar, UDP-N,N′-diacetylbacillosamine (UDP-diNAcBac). Previously, this UDP-sugar has been implicated in the pgl pathway of Campylobacter jejuni. Here we report the overexpression, purification, and biochemical characterization of the A. baumannii enzymes WeeK, WeeJ, and WeeI that are responsible for the production of UDP-diNAcBac. We also demonstrate the function of the phosphoglycosyltransferase (WeeH), which transfers the diNAcBac moiety to undecaprenyl-phosphate. UDP-diNAcBac biosynthesis in A. baumannii is also directly compared to the homologous pathways in the pathogens C. jejuni and Neisseria gonorrhoeae. This work demonstrates for the first time the ability of A. baumannii to generate the highly-modified, UDP-diNAcBac nucleotide sugar found previously in other bacteria adding to the growing list of pathogens that assemble glycoconjugates including bacillosamine. Additionally, characterization of these pathway enzymes highlights the opportunity for investigating the significance of highly-modified sugars in bacterial pathogenesis.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM097241)en_US
dc.description.sponsorshipNational Institute of General Medical Sciences (U.S.) Biotechnology Training Program (T32-GM08334)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.abb.2013.05.011en_US
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_US
dc.sourcePMCen_US
dc.titleBiosynthesis of UDP-N,N′-diacetylbacillosamine in Acinetobacter baumannii: Biochemical characterization and correlation to existing pathwaysen_US
dc.typeArticleen_US
dc.identifier.citationMorrison, Michael J., and Barbara Imperiali. “Biosynthesis of UDP-N,N′-Diacetylbacillosamine in Acinetobacter Baumannii: Biochemical Characterization and Correlation to Existing Pathways.” Archives of Biochemistry and Biophysics 536, no. 1 (August 2013): 72–80.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.mitauthorMorrison, Michael James
dc.contributor.mitauthorImperiali, Barbara
dc.relation.journalArchives of Biochemistry and Biophysicsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsMorrison, Michael J.; Imperiali, Barbaraen_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-5749-7869
mit.licensePUBLISHER_CCen_US


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