A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue
Author(s)Khan, Mustafa; Georgiou, John; Luyben, Thomas T.; Roder, John C.; Okamoto, Kenichi; Rivera Fuentes, Pablo Marcelo; Wrobel, Alexandra T.; Zastrow, Melissa L.; Lippard, Stephen J.; ... Show more Show less
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Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn²⁺ in lysosomes of HeLa cells, endogenous Zn²⁺ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy.
DepartmentMassachusetts Institute of Technology. Department of Chemistry
Royal Society of Chemistry, The
Rivera-Fuentes, Pablo; Wrobel, Alexandra T.; Zastrow, Melissa L.; Khan, Mustafa; Georgiou, John; Luyben, Thomas T.; Roder, John C.; Okamoto, Kenichi and Lippard, Stephen J. "A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue." Chemical Science 2015, 6 (January 2015): 1944-1948 © 2015 The Royal Society of Chemistry
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