| dc.contributor.author | Kamer, Kimberli J | |
| dc.contributor.author | Deerinck, Thomas J | |
| dc.contributor.author | Ellisman, Mark H | |
| dc.contributor.author | Mootha, Vamsi K | |
| dc.contributor.author | Lam, Stephanie Shih-Min | |
| dc.contributor.author | Martell, Jeffrey Daniel | |
| dc.contributor.author | Ting, Alice Y | |
| dc.date.accessioned | 2017-07-11T13:04:21Z | |
| dc.date.available | 2017-07-11T13:04:21Z | |
| dc.date.issued | 2014-11 | |
| dc.date.submitted | 2014-03 | |
| dc.identifier.issn | 1548-7091 | |
| dc.identifier.issn | 1548-7105 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/110613 | |
| dc.description.abstract | APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is far more active in cells, enabling the use of electron microscopy to resolve the submitochondrial localization of calcium uptake regulatory protein MICU1. APEX2 also permits superior enrichment of endogenous mitochondrial and endoplasmic reticulum membrane proteins. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (DP1 OD003961) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Nature Publishing Group | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1038/nmeth.3179 | en_US |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
| dc.source | PMC | en_US |
| dc.title | Directed evolution of APEX2 for electron microscopy and proximity labeling | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Lam, Stephanie S; Martell, Jeffrey D; Kamer, Kimberli J et al. "Directed evolution of APEX2 for electron microscopy and proximity labeling." Nature Methods 12, 1: 51–54 (January 2015) © 2015 Nature America, Inc | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
| dc.contributor.mitauthor | Lam, Stephanie Shih-Min | |
| dc.contributor.mitauthor | Martell, Jeffrey Daniel | |
| dc.contributor.mitauthor | Ting, Alice Y | |
| dc.relation.journal | Nature Methods | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Lam, Stephanie S; Martell, Jeffrey D; Kamer, Kimberli J; Deerinck, Thomas J; Ellisman, Mark H; Mootha, Vamsi K; Ting, Alice Y | en_US |
| dspace.embargo.terms | N | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0002-2687-3470 | |
| dc.identifier.orcid | https://orcid.org/0000-0002-8277-5226 | |
| mit.license | PUBLISHER_POLICY | en_US |
| mit.metadata.status | Complete | |
