Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays
Author(s)Carter, Bradley S.; Cortés-Campos, Christian; McCammon, Jasmine M.; Chen, Xiao; Sive, Hazel L
MetadataShow full item record
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
DepartmentMassachusetts Institute of Technology. Department of Biology
Mary Ann Liebert, Inc
Carter, Bradley S. et al. “Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays.” Zebrafish 14, 1 (February 2017): 73–76 © 2017 Mary Ann Liebert, Inc
Final published version