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Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays

Author(s)
Carter, Bradley S.; Cortés-Campos, Christian; McCammon, Jasmine M.; Chen, Xiao; Sive, Hazel L.
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.

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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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Abstract
Advances in genome-editing technology have made creation of zebrafish mutant lines accessible to the community. Experimental validation of protein knockout is a critical step in verifying null mutants, but this can be a difficult task. Absence of protein can be confirmed by Western blotting; however, this approach requires target-specific antibodies that are generally not available for zebrafish proteins. We address this issue using in vitro translation assays, a fast and standard procedure that can be easily implemented.
Date issued
2016-08
URI
http://hdl.handle.net/1721.1/111152
Department
Massachusetts Institute of Technology. Department of Biology
Journal
Zebrafish
Publisher
Mary Ann Liebert, Inc
Citation
Carter, Bradley S. et al. “Validation of Protein Knockout in Mutant Zebrafish Lines Using In Vitro Translation Assays.” Zebrafish 14, 1 (February 2017): 73–76 © 2017 Mary Ann Liebert, Inc
Version: Final published version
ISSN
1545-8547
1557-8542

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