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dc.contributor.authorCoe, Allison
dc.contributor.authorGhizzoni, Julie
dc.contributor.authorLeGault, Kristen Nicole
dc.contributor.authorBiller, Steven
dc.contributor.authorRoggensack, Sara
dc.contributor.authorChisholm, Sallie W
dc.date.accessioned2017-09-22T15:20:28Z
dc.date.available2017-09-22T15:20:28Z
dc.date.issued2016-07
dc.date.submitted2016-03
dc.identifier.issn0024-3590
dc.identifier.issn1939-5590
dc.identifier.urihttp://hdl.handle.net/1721.1/111625
dc.description.abstractProchlorococcus is the smallest oxygenic phototroph in the ocean, where it can be found in great abundance throughout the euphotic zone in mid-latitude waters. Populations of this picocyanobacterium have been observed below the euphotic zone, but the viability of these cells is unclear. To explore the tolerance of Prochlorococcus to extended light-deprivation, we subjected multiple strains of Prochlorococcus to varying periods of darkness and examined their ability to recover when placed back in the light. Some strains recovered after 35 h of darkness while others could not; this variability was not related to whether the strains were members of high- or low- light adapted ecotypes. The presence of a marine heterotroph, Alteromonas macleodii MIT1002, in the cultures extended their ability to survive prolonged darkness, in the most extreme case by 11 d. This could be attributed at least in part to the reduction of hydrogen peroxide in co-cultures, consistent with known roles of “helper bacteria” in detoxifying hydrogen peroxide, and this effect could be mimicked to some degree by the addition of a known hydrogen peroxide quencher, sodium pyruvate. The addition of glucose alone to the cultures provided marginal enhancement, but when both pyruvate and glucose were added together, all strains were able to survive longer in darkness than they were with only the heterotroph added. Thus, it appears that Prochlorococcus dark-survival depends on a multitude of factors. Limited analyses of Synechococcus suggest that its dark-survival capacity is longer than that of Prochlorococcus, for reasons that are not yet clear.en_US
dc.description.sponsorshipGordon and Betty Moore Foundation (Grant GBMF495)en_US
dc.description.sponsorshipSimons Foundation (LIFE-337262)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (OCE-1153588)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (OCE-1356460)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (DBI-0424599)en_US
dc.language.isoen_US
dc.publisherAssociation for the Sciences of Limnology and Oceanographyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/lno.10302en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourceProf. Chisholm via Anne Grahamen_US
dc.titleSurvival of Prochlorococcus in extended darknessen_US
dc.typeArticleen_US
dc.identifier.citationCoe, Allison et al. “Survival ofProchlorococcusin Extended Darkness.” Limnology and Oceanography 61, 4 (May 2016): 1375–1388 © 2016 Association for the Sciences of Limnology and Oceanographyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Civil and Environmental Engineeringen_US
dc.contributor.approverChisholm, S. W.en_US
dc.contributor.mitauthorCoe, Allison
dc.contributor.mitauthorGhizzoni, Julie
dc.contributor.mitauthorLeGault, Kristen Nicole
dc.contributor.mitauthorBiller, Steven
dc.contributor.mitauthorRoggensack, Sara
dc.contributor.mitauthorChisholm, Sallie W
dc.relation.journalLimnology and Oceanographyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsCoe, Allison; Ghizzoni, Julie; LeGault, Kristen; Biller, Steven; Roggensack, Sara E.; Chisholm, Sallie W.en_US
dspace.embargo.termsNen_US
dc.identifier.orcidhttps://orcid.org/0000-0002-2638-823X
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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