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Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates

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dc.contributor.author Acker, Leah Christine
dc.contributor.author Pino, Erica N.
dc.contributor.author Boyden, Edward
dc.contributor.author Desimone, Robert
dc.date.accessioned 2017-11-20T18:03:51Z
dc.date.available 2017-11-20T18:03:51Z
dc.date.issued 2017-10
dc.identifier.issn 1940-087X
dc.identifier.uri http://hdl.handle.net/1721.1/112242
dc.description.abstract This protocol describes a large-volume illuminator, which was developed for optogenetic manipulations in the non-human primate brain. The illuminator is a modified plastic optical fiber with etched tip, such that the light emitting surface area is > 100x that of a conventional fiber. In addition to describing the construction of the large-volume illuminator, this protocol details the quality-control calibration used to ensure even light distribution. Further, this protocol describes techniques for inserting and removing the large volume illuminator. Both superficial and deep structures may be illuminated. This large volume illuminator does not need to be physically coupled to an electrode, and because the illuminator is made of plastic, not glass, it will simply bend in circumstances when traditional optical fibers would shatter. Because this illuminator delivers light over behaviorally-relevant tissue volumes (≈ 10 mm 3 ) with no greater penetration damage than a conventional optical fiber, it facilitates behavioral studies using optogenetics in non-human primates. en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant NIH 2R44NS070453-03A1) en_US
dc.description.sponsorship National Institutes of Health (U.S.) (Grant NIH EY017292) en_US
dc.publisher MyJoVE Corporation en_US
dc.relation.isversionof http://dx.doi.org/10.3791/56330 en_US
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. en_US
dc.source Journal of Visualized Experiments (JOVE) en_US
dc.title Large Volume, Behaviorally-relevant Illumination for Optogenetics in Non-human Primates en_US
dc.type Article en_US
dc.identifier.citation Acker, Leah C et al. “Large Volume, Behaviorally-Relevant Illumination for Optogenetics in Non-Human Primates.” Journal of Visualized Experiments 128 (October 2017) © 2017 Journal of Visualized Experiments en_US
dc.contributor.department McGovern Institute for Brain Research at MIT en_US
dc.contributor.department Harvard University--MIT Division of Health Sciences and Technology en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Biology en_US
dc.contributor.department Massachusetts Institute of Technology. Media Laboratory en_US
dc.contributor.department Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences en_US
dc.contributor.mitauthor Acker, Leah Christine
dc.contributor.mitauthor Pino, Erica N.
dc.contributor.mitauthor Boyden, Edward
dc.contributor.mitauthor Desimone, Robert
dc.relation.journal Journal of Visualized Experiments en_US
dc.identifier.mitlicense PUBLISHER_POLICY en_US
dc.eprint.version Final published version en_US
dc.type.uri http://purl.org/eprint/type/JournalArticle en_US
eprint.status http://purl.org/eprint/status/PeerReviewed en_US
dc.date.updated 2017-11-02T18:42:19Z
dspace.orderedauthors Acker, Leah C; Pino, Erica N.; Boyden, Edward S.; Desimone, Robert en_US
dspace.embargo.terms N en_US
dc.identifier.orcid https://orcid.org/0000-0002-5534-6020
dc.identifier.orcid https://orcid.org/0000-0002-4019-7042
dc.identifier.orcid https://orcid.org/0000-0002-0419-3351
dc.identifier.orcid https://orcid.org/0000-0002-5938-4227


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