dc.contributor.author | Murano, Tatsuro | |
dc.contributor.author | Najibi, Mehran | |
dc.contributor.author | Paulus, Geraldine L. C. | |
dc.contributor.author | Adiliaghdam, Fatemeh | |
dc.contributor.author | Valencia-Guerrero, Aida | |
dc.contributor.author | Selig, Martin | |
dc.contributor.author | Wang, Xiaofei | |
dc.contributor.author | Jeffrey, Kate | |
dc.contributor.author | Lassen, Kara G. | |
dc.contributor.author | Irazoqui, Javier E. | |
dc.contributor.author | Xavier, Ramnik Joseph | |
dc.date.accessioned | 2017-12-07T16:35:36Z | |
dc.date.available | 2017-12-07T16:35:36Z | |
dc.date.issued | 2017-07 | |
dc.date.submitted | 2017-10 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/112630 | |
dc.description.abstract | Understanding the transcription factors that modulate epithelial resistance to injury is necessary for understanding intestinal homeostasis and injury repair processes. Recently, transcription factor EB (TFEB) was implicated in expression of autophagy and host defense genes in nematodes and mammalian cells. However, the in vivo roles of TFEB in the mammalian intestinal epithelium were not known. Here, we used mice with a conditional deletion of Tfeb in the intestinal epithelium (Tfeb[superscript ΔIEC]) to examine its importance in defense against injury. Unperturbed Tfeb[superscript ΔIEC] mice exhibited grossly normal intestinal epithelia, except for a defect in Paneth cell granules. Tfeb[superscript ΔIEC] mice exhibited lower levels of lipoprotein ApoA1 expression, which is downregulated in Crohn's disease patients and causally linked to colitis susceptibility. Upon environmental epithelial injury using dextran sodium sulfate (DSS), Tfeb[superscript ΔIEC] mice exhibited exaggerated colitis. Thus, our study reveals that TFEB is critical for resistance to intestinal epithelial cell injury, potentially mediated by APOA1. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant DK097485) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant DK43351) | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (Grant AI109725) | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1038/s41598-017-14370-4 | en_US |
dc.rights | Creative Commons Attribution 4.0 International | en_US |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |
dc.source | Nature | en_US |
dc.title | Transcription factor TFEB cell-autonomously modulates susceptibility to intestinal epithelial cell injury in vivo | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Murano, Tatsuro et al. “Transcription Factor TFEB Cell-Autonomously Modulates Susceptibility to Intestinal Epithelial Cell Injury in Vivo.” Scientific Reports 7, 1 (October 2017): 13938 © 2017 The Author(s) | en_US |
dc.contributor.department | Institute for Medical Engineering and Science | en_US |
dc.contributor.mitauthor | Xavier, Ramnik Joseph | |
dc.relation.journal | Scientific Reports | en_US |
dc.eprint.version | Final published version | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dc.date.updated | 2017-12-06T20:06:33Z | |
dspace.orderedauthors | Murano, Tatsuro; Najibi, Mehran; Paulus, Geraldine L. C.; Adiliaghdam, Fatemeh; Valencia-Guerrero, Aida; Selig, Martin; Wang, Xiaofei; Jeffrey, Kate; Xavier, Ramnik J.; Lassen, Kara G.; Irazoqui, Javier E. | en_US |
dspace.embargo.terms | N | en_US |
mit.license | PUBLISHER_CC | en_US |