| dc.contributor.author | Maji, Basudeb | |
| dc.contributor.author | Shoulders, Matthew D | |
| dc.contributor.author | Choudhary, Amit | |
| dc.contributor.author | Moore, Christopher | |
| dc.contributor.author | Zetsche, Bernd | |
| dc.contributor.author | Volz, Sara E. | |
| dc.contributor.author | Zhang, Feng | |
| dc.date.accessioned | 2017-12-13T15:31:24Z | |
| dc.date.available | 2017-12-13T15:31:24Z | |
| dc.date.issued | 2016-10 | |
| dc.date.submitted | 2016-05 | |
| dc.identifier.issn | 1552-4450 | |
| dc.identifier.issn | 1552-4469 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/112729 | |
| dc.description.abstract | Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation. | en_US |
| dc.publisher | Nature Publishing Group | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1038/NCHEMBIO.2224 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en_US |
| dc.source | PMC | en_US |
| dc.title | Multidimensional chemical control of CRISPR–Cas9 | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Maji, Basudeb et al. "Multidimensional chemical control of CRISPR–Cas9." Nature Chemical Biology 13 (January 2017): 9-11 © 2016 The Author(s) | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
| dc.contributor.department | McGovern Institute for Brain Research at MIT | en_US |
| dc.contributor.mitauthor | Moore, Christopher | |
| dc.contributor.mitauthor | Zetsche, Bernd | |
| dc.contributor.mitauthor | Volz, Sara E. | |
| dc.contributor.mitauthor | Zhang, Feng | |
| dc.relation.journal | Nature Chemical Biology | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dc.date.updated | 2017-12-12T18:48:39Z | |
| dspace.orderedauthors | Maji, Basudeb; Moore, Christopher L; Zetsche, Bernd; Volz, Sara E; Zhang, Feng; Shoulders, Matthew D; Choudhary, Amit | en_US |
| dspace.embargo.terms | N | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0003-2782-2509 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
