Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells
Author(s)Kind, Jop; Pagie, Ludo; de Vries, Sandra S.; Nahidiazar, Leila; Dey, Siddharth S.; Bienko, Magda; Zhan, Ye; Lajoie, Bryan; de Graaf, Carolyn A.; Amendola, Mario; Jalink, Kees; Dekker, Job; van Oudenaarden, Alexander; van Steensel, Bas; Fudenberg, Geoffrey; Imakaev, Maksim Viktorovich; Mirny, Leonid A; ... Show more Show less
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Mammalian interphase chromosomes interact with the nuclear lamina (NL) through hundreds of large lamina-associated domains (LADs). We report a method to map NL contacts genome-wide in single human cells. Analysis of nearly 400 maps reveals a core architecture consisting of gene-poor LADs that contact the NL with high cell-to-cell consistency, interspersed by LADs with more variable NL interactions. The variable contacts tend to be cell-type specific and are more sensitive to changes in genome ploidy than the consistent contacts. Single-cell maps indicate that NL contacts involve multivalent interactions over hundreds of kilobases. Moreover, we observe extensive intra-chromosomal coordination of NL contacts, even over tens of megabases. Such coordinated loci exhibit preferential interactions as detected by Hi-C. Finally, the consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3. These results highlight fundamental principles of single-cell chromatin organization.
DepartmentMassachusetts Institute of Technology. Department of Physics
Kind, Jop et al. “Genome-Wide Maps of Nuclear Lamina Interactions in Single Human Cells.” Cell 163, 1 (September 2015): 134–147 © 2015 Elsevier Inc
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