Organization of the Mitotic Chromosome
Author(s)Naumova, Natalia; Zhan, Ye; Lajoie, Bryan R.; Dekker, Job; Imakaev, Maksim Viktorovich; Fudenberg, Geoffrey; Mirny, Leonid A; ... Show more Show less
MetadataShow full item record
Mitotic chromosomes are among the most recognizable structures in the cell, yet for over a century their internal organization remains largely unsolved. We applied chromosome conformation capture methods, 5C and Hi-C, across the cell cycle and revealed two alternative three-dimensional folding states of the human genome. We show that the highly compartmentalized and cell-type-specific organization described previously for non-synchronous cells is restricted to interphase. In metaphase, we identify a homogenous folding state, which is locus-independent, common to all chromosomes, and consistent among cell types, suggesting a general principle of metaphase chromosome organization. Using polymer simulations, we find that metaphase Hi-C data are inconsistent with classic hierarchical models, and is instead best described by a linearly-organized longitudinally compressed array of consecutive chromatin loops.
DepartmentInstitute for Medical Engineering and Science; Massachusetts Institute of Technology. Department of Physics
American Association for the Advancement of Science (AAAS)
Naumova, Natalia et al. "Organization of the Mitotic Chromosome." Science 342, 6161 (November 2013): 948-953 © 2013 American Association for the Advancement of Science
Author's final manuscript